不同能量等级射频消融对半月板损伤的消融效应研究外科学专业论文.docxVIP

Study Study On The Ablation Effect Of Different Energy Levels Of Radio Frequency Ablation Systems On Torn Meniscus Abstract：ObjectiveTo study on the ablation effect of processing meniscus with different energy levels of radio frequency．Methods： taking 3 0 pieces of flesh pig knuckles，use a marking pen to make a longitudinal mark of 1．5cm long in the middle of the medial meniscus body,and then use sharp—pointed scalpel(拌1 1)to cut through the meniscus along the marked line to the surface of tibial cartilage to make meniscal tear model and divide them into five groups randomly,including four experimental groups and one control groupUse bipolar radio frequency gasification system under simulation arthroscopy at room temperature，(ArtroCare company)，and set it to the ablation file， according to different energy levels of file 2,4，6，8to process corresponding experimental group for 5 seconds，while setting a control group．Divide the processed specimen of each group into four parts after general morphologic observation：A，HE staining for histomorphology observation，and calculate the rate of empty cell；b，immunologic tissue staining observation and grading based on trisection system semiquantitative analysis；C，use FCM to calculate cell death rate；d，using scanning electron microscope(SEM)to observe the surface morphology and smoothness of fibrous cartilage．Results：With the increase of energy,the ablation effect of bipolar radio frequency is enhanced，gross 一3一 万方数据 observation：normal observation：normal meniscal surface is smooth with bright white color,it touches strong and elastic，after each group is processed，the color becomes yellowish with the energy level increasing，but the incision and the surface area around it is smooth and bright，cutting marks may occur with greater energy levels，burnt brown spots are visible after 8-profile processing，and the area around the processed edge is hard．HE staining： normal meniscus tissue is composed of mass arrangement of c