蓖麻下胚轴再生体系的建立及遗传转化初探作物专业论文.docxVIP

侵染率可达到21．67％。 侵染率可达到21．67％。 关键词：蓖麻；组织培养；下胚轴；丛生芽；农杆菌介导 万方数据 The The estabHshment of castor hypoco留l№genera缅n system and the preHminary stlIdy on its genetic traⅡsforma戗on Abstract IIl the eXperi玎1ent，we chose t11e matllre caStor seeds嬲raw mat翻als to study the variolls factorS inVolved in vi仃o cuh鹏process syst锄tically with hypocotyl觞the exphnt．Then we e)【plored preliIninarily the genetic仃anSfomlation system of castor hypocotyl e)【plant mediated by agrobacteri啪．The瑚ins results were髂f0Uows： 1．The best me也od for st砸uzing of raw mat缸als：firstly也e Sheus of c勰tor seeds were removed粗d nushed响h s10wly water now 30min．Under st函le condition，the seeds were djsin免cted 30sec 17l，ith me Vol衄1e丘．action of 75％alcohol姐d nushed 3~5 tiInes winl sterile water；n肋soaked inⅡle m嬲s丘action of 0．1％HgCl2 3miIl，nushed 6~8 tiInes with steriIe water．Under也e印prop缸e cuItl鹏condition，we c矾get a ve口10w cont础ation rate aIld lligher g锄iIlation rate． 2．On nle昏团船tee of extrernely low contamination rate，tlle hardness of pre-culttlred seed rnedium w舔optiⅡlized，锄d the gem恤ation rate of the seedS could be improved wh％ the agar co∞ent训on w舔99儿． 3．By usingⅡ1e nlodi丘ed culture mcdium of MS+209／L sucrose and 99如吗ar with pH Vahle 5．6～5．8，buds，addition of 100mg／L觞corbic acid caIl bc e彘ctive material for pre呦tion alld con仃ol ofbro、ⅣIling，when the concentration of TDZ w淞O．3mg几，just i110culated explants under suitabk conditioIls and dar!bess pre仃cannent for 6d and t11en 仃a11sfeIred to the Hght血e璐毋w舔1 ooO～1 5001)【，dayJ，night cycle 1 6／8h a day llnder吐1e condition ofcultiVation，cespitose bud induction e丘．ect w硒best． 4．For t11e iIlduction of adVentitiollS bud elongatio玛the in伊edients of廿1e best cunure nledium iIlcluded，1／2MS+o．2m雩皿GA3+o．1m鲫脚209／L sucrose+99／L agar withpH Value 5．60．8．Add 100 mg／L aScorbic acid，followil鸣ttle genera|曲n cycle by original eveDr 2 to 3 weeks吼lbcultll】re time mo every 10 days subcukure o雎tinle can be s蛳丘cantly re山lced the rate ofthe gIass mat商alS． 5．The screening results of tlle beSt