创伤性兔膝骨关节炎关节软骨细胞凋亡基质金属蛋白酶组织抑制因子3timp3及神经元型一氧化氮合酶nosⅰ变化的实验研究中医骨伤科专业论文.docxVIP

ABSTRACTObjecti ABSTRACT Objecti ve：To characteri the eff‘ects of carti Iage ceI apoptosis、 tissue inbibitor of璜ot8iloprotej ease一3 and Neurona{nit ric oXide Synthase in the pathogenesis of osteoarthritis。Methods：E{ghteen heal thy chinchiIla rabbits(female and male be in haif)are di vided into groups randomly(without conscious choice)，9 in each group，Use Th{opental sodium 30mg／kg to anesthetic of intr韪鼙eritoneal+Then fetch the inner knee joil3ts of two Sides in germ condjtion about 2em i 1 ength。Explore 礴ith hacked eyes，if there genera{d{sease，USe ophthaIme}ogyical surgical sci and oi’f the hack and}’Fen{ r10SS-1 igamenttlm。 the i nside sub—j igamefi Luin，the compIete inside mQni sci tf}|-0rm；l modej．Meanwhiie，to ebeserve the DNA content and¨mp一：{，、()Sj pesf Li strength during 6 and#weeks by the method ol¨UOFescence{滋ti vi ted eel}analysis and jmmunohjstochemlcal+ResuI ls：,Neuronal ni tric OXide Synthase：No remarkabIe di i‘ferences among each group hp si h week。but there obviOUS di fl’ercnces between the iutet|e翻1agen and other groups at the eighth week：tissue inhibitar of riletaI opretei ease-：{： There remarkable differenees among each groups：There obvious differences between normal group and injured groups weli hyperplaria hypertrohy group and injured group：There obvious di fferences among other the di fferences great when eighth week：Diffefences oceured mainl between nortllal group and coHagen rinjuring group、normal group and hyperpIarin hyperl rohy group、 normai groupand injured group、coJ Iagen{njuri ng group rkf hyperplari}l hypertrohy group、COll agen i njuri groupand njured group，no obvious differences coujd be observed between hyperpl ari}t hypertrnhy anti injured group。Duri ng these si and oigh weeks。l hp exprussi{ln flr ti i nhih{tor otl i]te{a|{oprotci n}|se一：； grea}Hlfl{lng{ht，normal group，but very anyiOUS among the ahnormill groups螺1)f}：A}l he eighth week，the of．DNA quite di sti ngui shed helween the nurmHI“n(1 abnormal groups．but the DNA