响应面法优化超声提取野葛茎中葛根素的工艺-广东农业科学.PDFVIP

广东农业科学 2015 年第 4 期 60 响应面法优化超声提取野葛 茎中葛根素的工艺 1,2 1 1 1 陈 帅 王慧竹 薛健飞 钟方丽 1.吉林化工学院化学与制药工程学院袁 吉林 吉林 132022 曰 2.沈阳药科大学药学院袁 辽宁 沈阳 110016冤 要 为了研究野葛茎中葛根素的超声提取工艺条件袁在单因素试验的基础上袁根据星点试验设计原 理袁选取提取温度尧乙醇 度尧超声时间和液料比作为自变量进行试验设计袁利用响应面法对结果进行分析遥 结 果表明院提取温度尧乙醇 度和超声时间对野葛根茎中葛根素的提取率均有显著影响袁最佳提取工艺条件为院 提取温度 57益尧 乙醇 度 69% 尧 超声时间45 min尧 液固比21颐1 袁 在此工艺条件下袁 葛根素提取率预测值为 3.532% 袁验证值为 3.524%遥 关键词 响应面法曰 超声曰 野葛根茎曰 葛根素 中图分类号 R284.2 文献标识码 A 文章编号 1004-874X 2015冤04-0060-07 Optimization process of ultrasonic-assisted extraction of puerarin from the stem of Pueraria lobata by response surface methodology 1,2 1 1 1 CHEN Shuai , WANG Hui-zhu , XUE Jian-fei , ZHONG Fang-li (1.School of Chemistry and Pharmace utical Engineering, Jilin Institute of Chemical Teconology, Jilin 132022, China; 2.Sc hool of Pharmacy, Shenyang Pharmaceutical Unive rs ity, Shenyang 110016, China) A bstract: The purpose of the study is to optimize the ultrasonic-assisted extraction process of puerarin from the stem of Pue raria lobata. T he extraction temperature, concentration of alcohol, ultrasonic time and ratio of liquid to solid were chosen as independent variables based on the single factor experiment, and the experimental program was arranged according to the principle of central composite design. The result was analyzed by response surface methodology. The results sh