双载蛋白对果蝇S2细胞吞噬大肠杆菌的影响.pdf

中国细胞生物学学报 Chinese Journal of Cell Biology 2017, 39(1): 52–57 DOI: 10.11844/cj cb.2017.01.0281 研究简报 双载蛋白对果蝇S2细胞吞噬大肠杆菌的影响 * 孙保贞 朱 斐 (浙江农林大学动物科技学院, 杭州311300) 摘要 该文研究了双载蛋白(amphiphysin, Amph)对果蝇S2细胞吞噬大肠杆菌(Escherichia coli, E. coli)的影响。在S2细胞吞入E. coli后, Amph mRNA 的表达水平明显升高; 但用Amph-siRNA 处理后的S2细胞, 其Amph蛋白质水平显著低于正常和吞噬E. coli 的S2细胞。透射电镜观察发现, S2细胞可以吞噬热灭活的E. coli, 且细胞内存在典型的吞噬小体, 而用Amph-siRNA处理的S2细胞 内则未发现吞噬小体, 说明Amph可能对吞噬体形成起关键作用。Amph-siRNA处理的S2细胞对E. coli的吞噬率显著低于未用Amph-siRNA处理的S2细胞, 说明敲低Amph会显著地抑制S2细胞对E. coli的吞噬率。上述结果表明, Amph在果蝇S2细胞吞噬E. coli 的过程中起重要的调节作用。 关键词 双载蛋白; 果蝇; S2细胞; 吞噬; 大肠杆菌 The Effect of Amphiphysin on the Phagocytosis of Escherichia coli by Drosophila S2 Cells Sun Baozhen, Zhu Fei* (College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China) Abstract The effect of amphiphysin (Amph) on the phagocytosis of Escherichia coli (E. coli) by Drosophila S2 cells was studied in this paper. The observation under electron microscopy showed that heat- inactivated E. coli was phagocytosed by S2 cells, and there were typical phagocytic bodies in the S2 cells. The phagocytic bodies were not found in the S2 cells treated with Amph-siRNA. After S2 cells phagocytosed E. coli, the mRNA level of Amph gene was significantly increased. However, the protein level of Amph in Amph-siRNA S2 cells was significantly lower than that of normal S2 cells and S2 cells which phagocytosed E. coli . These results indicated that Amph plays an important role in the process of phagocytosis of E. coli in Drosophila S2 cells. Keywords amphiph