富硒绿茶糖蛋白的分离纯化及结构初步研究生物化学与分子生物学专业论文.docxVIP

Abstract Abstract 上海师范大学硕士学位论文 Abstract Se—enriched green tea produced in Enshi was used as a raw material in this paper，in which distribution of selenium was investigated．In addition，selenium ell-fiched glycoprotein from green tea were successively ex仃acted，separated and purified，and the structure information was analyzed．The researches provide the theoretical basis for the development of selenium enriched green tea．The main results ofthis paper were as follows： The distribution of Selenium in Se—enriched green tea was investigated，and the sequential ectraction of protein according to Osborne method and the analysis of selenium content were developed．Moreover，amnio acid composition and molecular weight distribution of the protein extracted from Se—enriched green tea were determined．In se-enriched tea，the organic selenium is 7 1．3％and most organic selenium was incorporated into protein；Water soluble protein and alkali soluble protein the main protein of the selenium binding protein of tea and the content of selenium were O．53 1,0．28 1 mg se／g protein respectly．The content of aspartic Acid，serine，glutamic acid and arginine is highly in the amino acid composition of alkali·soluble proteins，alcohol-soluble protein，water．soluble protein．The molecular weight(Mw)distribution of four kinds of protein were between 958，1 108，1 14．8，1 135 and 3 1 83757，2562665，5782584，3420 Da，respectively． TSBGP was sequentially separated and purified from water．soluble protein by several methods as follow：ammonium sulfate precipitation，DEAE Sepharose Fast Flow ion exchanging chromatography，and Sephacryl 400 molecular excluding chromatography．Results showed that，the rate of protein production was 6．4％when 60％of ammonium sulfate was selected for precipitating the water-soluble protein．Three fractions(TSBGP 1-1，TSBGP 2．1 and TSBGP 3．1 1 were purified by using DEAE Sepharose Fast Flow and Sephacryl 400，and the Selenium content in glycoprotein was 1．25mg，1．075mg and 1．849mg，respectively．Moreover