骨髓间充质干细胞对脑缺血大鼠轴突生长与AKT2fGSK-3β2fCRMP-2表达的影响.pdfVIP

BMSCs 可促进脑缺 血大鼠的神 经功能的恢 复，其机 制可能通过 活化 AKT/GSK-3β/CRMP-2 信号通道起作用。 【关键词】骨髓间充质干细胞,局灶性脑缺血模型,神经功能,磷脂酰肌醇激酶-3/丝 苏氨酸蛋白激酶通路，糖原合成酶激酶-3 β，生长相关蛋白 43 5 万方数据 Effects of Bone marrow-derived mesenchymal stem cells on the axonal outgrowth and expression of AKT/GSK-3β/CRMP-2 in rat brain after cerebral ischemia Abstract Object: It has been generally accepted that Bone mesenchymal stem cells (BMSCs) can improve neurological deficit recovery following stroke. The aim of the research is to investigate the effects of BMSCs on the axonal outgrowth and expression of AKT/GSK-3β/CRMP-2 signaling pathway in rat cortical neurons followed MCAO. We sought to potential mechanisms that BMSCs effect on axonal outgrowth in non-contact system. Methods: 1. BMSCs from SD rats were cultured and identified in vitro. 2. The SPF 7 to 9 weeks male SD rats were randomly divided into four groups: sham group, MCAO group, MCAO+BMSCs group and MCAO+BMSCs+LY29004 group (12 rats in each). 3. The acute transient focal cerebral ischemia-reperfusion model was established by modified thread-occlusion method. The sham group underwent the same procedure without inserting the suture. LY294002 was injected into the lateral ventricle of rats 1 hour before MCAO. BMSCs (1×106 in 1ml PBS) were injected into the tail vein of rats 24h after MCAO . 4. Modified neurological severity score(mNSS) and modified adhesive removal test were performed before and at 1,3,7,10 and