肺癌染色体和dna甲基化异常改变及其诊断价值的研究卫生毒理学专业论文.docxVIP

第三军医大学博士研究生论文 第三军医大学博士研究生论文 肺癌染色体和DNA甲基』匕异常改变及其诊断价值的研究 英文缩写一览表 缩写 英文全文 中文 FISH Fluorescence in situ hybridization 荧光原位杂交 琢ISH Interphase—FISH 间期细胞荧光原位杂交 SKY Spectral karyotyping 频谱式染色体核型分析 CGH Comparative genomic hybridization 比较基因组杂交 MSP Methylation—specific PCR 甲基化特异性PCR Methylation-sensitive 甲基化敏感性 MS．AP．PCR Arbitrarily primed PCR 随机引物PCR SCC Squamous cell carcinoma 鳞癌 ADC Adenocarcinoma 腺癌 ASC Adenosquamou carcinoma 腺鳞癌 LCLC Large cell lung cancel 大细胞肺癌 SCLC Small cell lung cancer 小细胞肺癌 FOB Fiberoptic bronchoscopy 纤维支气管镜 O-·6··methylguanine·-DNA 06．甲基鸟嘌呤．DNA MGbfr methyltransferase 甲基转移酶基因 p16 Cyclin dependent kinase 2A p16基因 FHH Fragile histidine triad gene FHlT基因 删C Bactedal artificia】chromosome 细菌人工染色体 第三军医大学博-L-z开究生论文 第三军医大学博-L-z开究生论文 肺癌染色体和DNA甲基化异常改变及其诊断价值的研究 Chromosome and DNA Methylation aberrations in Chinese lung carcinomas and their potential in clinical detection Abstrac叶 Lung carcinomas are now the leading cause of cancer mortality worldwide among both men and women．In china，this neoplasm has become the most fatal malignant disease．with its incidence keeping rising in the last two decades． There are three strategies to reduce the rate ofmortality oflung cancer：prevention， early detection and advances in treatment．To date，early dew．ion，especially molecular diagnosis represents the most promising strategy． With the understanding of the genetic basis of lung carcinogenesis，there raises the hope that molecular markers could be of great use to detect lung cancer at the earliest stages of this disease．It is clearly important to develop effective molecular diagnostic methods to enable early detection and therapeutic intervention in lung cancer． In the present study,IFISH rinterphase fluorescence加situ hybridization)was applied to analyze the molecular cytogenetic alterations in sputum cells from lung cancer patients and cells from two SV40 L·T immortalized human bronchial epithelial cell lines M and Y，while spectral karyotyping(SKY)Was perform