热打击对单层肠上皮Caco-2细胞屏障功能的影响.PDFVIP

　 军 杂 20 13年6月1 日　第38卷　第6期 472 解放 医学 志　 论　著 热打击对单层肠上皮Caco-2细胞屏障功能的影响 肖桂珍，袁芳芳，古正涛，刘志锋，张亚历，苏磊 [摘要]　目的　研究热打击对肠黏膜屏障上皮细胞间紧密连接及凋亡的影响。方法　采用肠上皮细胞株Caco-2建 立肠屏障模型，检测经37~4 3 ℃热打击后细胞的单层跨膜电阻抗(TEER)值及对大分子物质辣根过氧化物酶(HRP) 的通 透性，采用Western bl otting检测occludin蛋白的表达水平，A nnex in V-FITC/PI流式细胞术检测细胞凋亡情况。结果　随 着热打击温度的升高，TEER值降低，HRP通透性增加，37 ℃热打击细胞的TEER值和HRP通透性与39 、4 1、4 3 ℃热打 击细胞差异均有统计学意义(P＜0.0 1) 。热打击温度从37 ℃到4 1℃，occludin蛋白的表达逐渐增加，于4 1℃达峰值，此后 逐渐下降。43 ℃时随热打击时间延长，occlu din蛋白表达逐渐降低。随热打击温度(37~43 ℃)升高，细胞凋亡逐渐增加 P＜ P＜ ( 0.05或 0.0 1) 。结论　热打击可造成肠上皮紧密连接的明显破坏，细胞凋亡增加，从而破坏肠屏障功能。 [关键词]　中暑；肠黏膜；细胞凋亡 [中图分类号]　R594. 12 [文献标志码]　A [文章编号]　0577-7402 (20 13)06-0472-04 Effect of heat stress on intestinal barrier function of human intestinal epithelial Caco-2 cells XIAO Gui-zhen 1 2 2 2 1* 2* , YUAN Fang-fang , GU Zheng-tao , LIU Zhi-feng , ZHANG Ya-li , SU Lei 1Key Laboratory of Gastroenterology of Guangdong Province, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China 2Key Laboratory of Hot Zone Trauma Care and Tissue Repair of PLA, Department of Intensive Care Unit, General Hospital of Guangzhou Command, Guangzhou 510010, China * Corresponding author. ZHANG Ya-li, E-mail: zyl41531@163.com; SU Lei, E-mail: slei_icu@163.com This work was supported by the grant from National Natural Science Foundation of China [Abstract]　Objective　To inv estigate th e h eat stress-indu ced dy sfuncti on of intestinal b arri er inclu ding intestinal tight junction and ap optosis of epith elial cell s. Methods　Human intestinal epith elial Caco-2 cell monolay ers, serv ing as th e intestinal b arrier model, w ere ex p osed to different temp erature (37-43 ℃) for designated time. Transepith elial el ectrical resistance (TEER) and