十字花科蔬菜基因组含量的测定与分析.PDFVIP

2011, 12( 1): 103106 JournalofP lantGenetic Resources 十字花科蔬菜基因组含量的测定与分析 李 蔚, 刘莉莎,李 仁, 郭仰东 (, 100193) : 以我国 14种重要十字花科蔬菜为材料, 利用流式细胞术 定基因组含量其中青苤蓝乌塌菜雪里蕻芥蓝的基 因组含量属首次报道本试验数据与国外文献提供的相关数据对比, 发现青萝卜结球甘蓝青花菜和根用芥菜的基因组含 量与已报道数值基本吻合, 而大白菜花椰菜的基因组含量值与报道数据存在差异造成同一物种基因组含量值差异的原因 可能是品种的不同, 也可能与生长环境或 定时参考标准选用等因素不同有关 : 十字花科蔬菜; 流式细胞术;基因组含量; 遗传变异 Analysis of G enom e Contents in Som e Cruciferous V egetables LIWe,i LI Lisha, LIRen, G O Yangdong (College of A griculture and B iotechnology, Ch inaA gricultural University, B eij ing 100193 ) Abstract: The genom e contents of 14 major cruciferous vegetables were measured. The genome contents of greenkohlrabi(B rassica oleracea L. var. caulorap a D. C. ) , savoy (Brassica chinensis var. rosularis Tsen et Lee. ), leafmustard (B rassica juncea var.m ulticeps Tsen etLee. ) and Chinese kale (Brassica al ogla ra Bailey) were firstly reported in the current study. Comparing the relevantdata and num erical of foreign literature provided, the genome contents of the green radish (Raphanus sativus Bailey) , cabbage (B rassica oleracea L. var. cap itata ), broccoli(B rassica. oleracea L. var. ita lica ) and rootmustard (B rassica j uncea Coss) were basically consistentw ith the reported values, the genome contents of the heading Chinese cabbage (B rassica camp estris L. var. cephalata Ts en et Lee) and cauliflower (B rassica oleracea L. var. otry tis L. ) were some different from the reported data. The reasonswhich cause the variation in genome content of the same specieswere possibly the difference ofbreed, the diverse grow ing environmentor the inconsistent reference standard. K ey w ord s: Cruciferous vegetables; Flow cytometry; Genom e content; Genom e variation ,