铁皮石斛内生真菌次生代谢产物研究.PDFVIP

热带亚热带植物学报 2019, 27( 1): 102 ~ 108 Journal of Tropical and Subtropical Botany 铁皮石斛内生真菌次生代谢产物研究 1 2 3* 徐迪 ，金日升 ，王海翔 (1. 芜湖职业技术学院，安徽 芜湖 241003 ；2. 合肥工业大学农产品生物化工教育部工程研究中心，合肥 230009 ；3. 中国药科大学工学院，南京 211198) 摘要：为了解铁皮石斛(Dendrobium officinale) 内生真菌Phyllosticta aristolochiicola 的次生代谢产物，从该真菌中分离得到15 个化合物，经波谱分析分别鉴定为N-methyl-2-pyrolidinone (1) 、环-(甘氨酸- L-脯氨酸) (2) 、环-(D-丙氨酸-L-脯氨酸) (3) 、环-(L- 缬氨酸-L-脯氨酸) (4) 、环-(L-亮氨酸-L-脯氨酸) (5) 、cyclo-(L-Leu-D-4-hydroxyprolinyl) (6) 、环-(L-苯丙氨酸-L-脯氨酸) (7) 、环-(L- 苯丙氨酸-L-4-羟基脯氨酸) (8)、环-(L-酪氨酸-L-脯氨酸) (9) 、环-(L-苯丙氨酸-L-亮氨酸) (10) 、啤酒甾醇 (11) 、对羟基苯乙醇 (12) 、对羟基苯乙酸 (13) 、(2S,3R)-1-(4-羟基苯基)丁烷-2,3-二醇 (14)和(2R,3S)-1-苯基丁烷-2,3-二醇 (15) 。采用MTS 法检测 抗肿瘤活性表明，化合物2 、10 和14 对HL-60、A-549、SMMC-7721、MCF-7 和SW-480 细胞株具有一定的抑制活性。 关键词：铁皮石斛；内生真菌；化学成分；抗肿瘤活性 doi: 10.11926/jtsb.3928 Study on Secondary Metabolites of Endophytic Fungus Phyllosticta aristolochiicola from Dendrobium officinale 1 2 3* XU Di , JIN Ri-sheng , WANG Hai-xiang (1. Wuhu Institute of Technology, Wuhu 241000, Anhui, China; 2. Engineering Research Center of Bioprocess Ministry of Education , Hefei University of Technology, Hefei 230009, China; 3. School of Engineering, China Pharmaceutical University, Nanjing 211198, China) Abstract: In order to understand the chemical constituents of endophytic fungus Phyllosticta aristolochiicola from Dendrobium officinale , fifteen compounds were isolated. On the basis of spectral data, their structures were identified as N-methyl-2-pyrolidinone (1), cyclo-(glycine-L-proline) (2), cyclo-(D-alanine-L-proline) (3), cyclo- (L-valine-L-proline) (4), cyclo-(L-leucine-L-proline) (5), cyclo-(L-leucine-D-4-hydroxyprolinyl) (6), cyclo-(L- phenylalanine-L-proline) (7), cyclo-(L-4-hydroxyproline-L-phenylalanin