阿托伐他汀对动脉粥样硬化大鼠apoe基因表达调控的影响内科学专业论文.docxVIP

Effect Effect of atorvastatin on ApoE gene expression in atherOsclerosis rats Abstract Objective：A large number of studies have indicated that the polymorphism of ApoE gene is associated with various diseases such as coronary heart disease and other diseases．Statins can reduce blood lipid and provide resistance against inflammation Using high fat diet building atherosclerosis rats model，and the model rats were given atorvastatin for the treatment，the determination of rat ApoE gene expression changes as well as the changes in LDL—C，HDL-C，TC，TG，C—reactive protein and atherosclerosis index．To investigate the relationship between the changes of ApoE gene expression and the statins．So as to guide the clinical intervention of high—risk genotypes of coronary heart disease，reduce the incidence of cardiovascular events，reduce the economic burden of the family．Methods：30 SD rats were divided into three groups：(A)control group(B)experimental group(C)intervention group：each of them is 1 0． A group were given basic feed，B，C group were fed with high fat diet to manufacture atherosclerosis rats．After modeling for 8 weeks，each group kill 2 rats and observe the atherosclero sis．from the 9th week C group rats were given atorvastatin 5mg／kg／d，A，B group were given the same amount of saline，gavage for 4 weeks．Then kill all the rats and Determination of TC，TG，LDL-C，HDL—C，C reactive protein in rats and the expression of ApoE in rat liver by real—time PCR，calculate the Atherosclerosis Index．Results：@Compared with the control group， experimental group and intervention group，serumTC，TG，LDL·C ．3一 万方数据 increased increased significantly(P0．0 1)．@The experimental group compared with the intervention group and intervention group，serum TC，TG， LDL—C decreased(P0．05)，and the HDL—C increased(P0．0 1) @Compared with the control group，the experimental group rats ApoE mRNA expression decreased(P0．05)； @the experimental group and the intervention group compared to intervention group rats PCR expres