稻瘟菌侵染初期宿主细胞微丝骨架及挥发性代谢物的研究微生物学专业论文.docxVIP

Abstract Abstract lIll l l ll l ll ll l l l l II III ＼1 806890 Abstract In plant-pathogen interaction,the infected host cells initiated a cascade of resistance—related events in order to prevent pathogen infection．After infected with the pathogen，not only the host cell cytoskeleton，but also the host’S metabolic pathways and metabolites changed．This research presented a detailed investigation of Magnaporthe grisea spore germination，appressorium formation and it’S successful infection of the host cells after inoculated in the rice leaf sheath，and the host cell’S microfilament re—organization and the changes of volatile metabolites of the rice leaf sheath during the early stages of disease development and showed difference between the compatible and incompatible interaction．The main results were as follows：1．Treatments of spore suspension with the microfilament inhibitor cytochalasin D(CD)indicated that 1-20ug／ml CD treatment had no effects on spore germination and appressorium formation ofM．grisea strain YOU 2．There is no difference in the spore germination，appressorium formation and successful infection of host cells of the M．grisea in resistant rice P8 1 6 and susceptible rice SE2 1 S．3．Inoculated with M grisea．the infected host cell microfilament was damaged，but the extent of damage of P8 1 6 was less than that in SEE2 1 S．4．Many of volatile metabolites of P8 1 6 inoculated with M．grisea were different from that with sterile water．The similar results were found in the volatile metabolites of SE21 S．The volatile metabolites were alSO different between P8】6 and SE2】S． Keyword：Rice，Magnaporthe grisea，Microfilaments，Inhibitor,Volatile metabolite III 福建师范大学张宇玲硕士学位论文 IV 中文文摘中文文摘 中文文摘 中文文摘 水稻是重要的粮食作物之一。稻瘟病是水稻的三大病害之一，严重影响水稻的 产量。为了更有效的控制这一病害的发生，我们在前人对其病原学、生理学、抗病 育种等方面的研究取得成绩的基础上，进一步研究不同的水稻在抵抗稻瘟病菌侵染 Lr◆， 中的差异。稻瘟病菌的侵染会对不同品种的水稻细胞骨架产生哪些不同的影响，稻 瘟病菌的侵染会对不同互作类型中的水稻的挥发性物质的释放产生哪些不同的影 响。 采用悬滴法检测不同浓度的细胞松弛素D对稻瘟病菌孢子的发芽以及附着胞的 形成的抑制影响。结果发现细胞松弛素D的浓度不高于2099／ml时，不会对稻瘟病 菌孢子的发芽及附着胞的形成产生抑制作用