小型凝胶渗透色谱净化系统在农残检测中的应用-上海舜宇恒平科学仪器.PDFVIP

《现代仪器与医疗》 2013 年 第 19 卷 第 2 期 检验检测 小型凝胶渗透色谱净化系统在农残检测中的应用 Application of miniaturized gel permeation chromatography (GPC) clean up system in the analysis of pesticide residues 摘  要   本文对小型凝胶渗透色谱净化（GPC ）系统在农残检测中的应用进行研究， 优化净化条件，包括净化柱选择和不同流动相比较。样品采用乙腈- 乙酸乙酯混合 溶剂均质提取，经GPC 净化后由气质联用仪（GC-MS ）检测。采用规格为2.0mm ×150mm 的GPC 净化柱，流动相为环己烷- 乙酸乙酯（1:1 ），流速0.1mL/min ，单 1,2 2 1 个样品净化时间在10min 以内，溶剂用量不到常规GPC 消耗量的10%，样品处理效 李 钧   蔡 强   黎 路 * 1,2 2 1 率提高2~4 倍。本方法平均加标回收率74.67%~109.20 ％，方法重现性良好，解决常 Li Jun Cai Qiang Li Lu 规GPC 净化应用中溶剂消耗量大的实际问题，可达到更为环保的效果。 （1.上海舜宇恒平科学仪器有限 关键词  凝胶渗透色谱 净化 溶剂消耗量 农残 公司  上海  200233；2.浙江 清华长三角研究院  嘉兴  Abstract The application of gel permeation chromatography (GPC) to the analysis of 314006） pesticide residues was studied. The clean up experimental parameters were optimized. (1.Shanghai Sunny Hengping Scientific Extraction of the pesticides with mixture of acetonitrile and ethyl acetate was carried instrument Co., Ltd. Shanghai out. The extracts were cleaned up by GPC and detected by GC-MS. Sample clean up 200233; 2.Yangtze Delta Region Insti- time was less than 10min with a flow rate of 0.1mL/min when using 2.0mm×150mm tute of Tsinghua University Zhejiang GPC clean up column and cyclohexane - ethyl acetate (1:1) mobile phase. The sample Jiaxing 314006) preparation time was shorted to 2 to 4 times, with less than 10% solvent consumption comparing to normal GPC. The average recovery rate was between 74.67% and 109.20% with high reproducibility. The probl