二十碳五烯酸甲酯标准样品的研制-吉林农业大学学报.PDFVIP

DOI :10.13327/j.j jlau.2006.01.028 　28 1 　　　　　　　 Vol.28 No.1　 　2006 2 Journal　of 　Jilin　Agricultural　University Feb.2006　 1 2 1 3 荣　会 , 胡秀丽 , 牟　峻 , 刘　丽 (1.吉林出入境检验检疫局, 长春 130062;2.吉林大学再生医学科学研究所, 长春 130021; 3.深圳出入境检验检疫局, 深圳518045) 　:以深海鱼油为基础原料, 将其皂化水解为游离脂肪酸, 经尿 — 甲醇包合粗分离后, 以硫酸— 甲醇甲 酯化, 再经硝酸银—硅胶柱层析精分离, 得到高纯度的二十碳五烯酸甲酯标准物质。 经定值检测, 确定其化合 物纯度≥98%。 :二十碳五烯酸甲酯;鱼油;尿 — 甲醇包合;硝酸银—硅胶柱层析 :O623.61　　　:A 　　　:1000-5684 (2006)01-0103-05 Development of Standard Samples of Eicosapentaenoic Acid Methyl Ester 1 2 1 3 RONG Hui , HU Xiu-li , MU Jun , LIU Li (1.JilinEntry-Exit Inspection and QuanrantineBureau, Changchun 130062, China;2.Institute of Re- generativeMedical Sciences, Jilin niversity, Changchun 130021, China;3.Shenzhen Entry-Exit In- spection and QuanrantineBureau, Shenzhen 518045, China) Abstract:Standard substance of eicosapentaenoic acid methyl ester made from a natural source of deep sea fish oil was first saponified in al aline medium , and separated by enclosing it with urea-methanol, and methylated with sulphuric acid-methanol and separated with silver nitrate-silica gel column by chro- matography.The obtained standard substance of eicosapentaenoic acid methyl ester was analyzed qualita- tively and quantitatively, and its purity was greater than or equal to 98%. Key words:eicosapentaenoic acid methyl ester;fish oil;urea-methanol enclosing;silver nitrate-silica gel column chromatography 　　 , EPA 。 。20 , , 、、、, 。(eicosapen- 。 taenoic acid , EPA)ω-3 ,