益母草饮片标准汤剂研究.docxVIP

益母草饮片标准汤剂研究 　　[摘要]建立益母草饮片标准汤剂质量评价标准。制备13批不同品质的益母草饮片标准汤剂，对盐酸益母草碱和盐酸水苏碱进行含量测定、计算其转移率、测定出膏率及pH、建立HPLC指纹图谱分析方法。结果13批益母草饮片标准汤剂中盐酸益母草碱和盐酸水苏碱转移率分别为300%～534%，670%～826%，干膏得率121%～183%，pH 587～622，并用中药色谱指纹图谱相似度评价系统软件进行指纹图谱分析，确定了12个共有峰，对13批益母草饮片标准汤剂分别进行相似度评价，其相似度均大于09。该研究中益母草饮片制备方法规范，指纹图谱相似度高，其方法精密度、稳定性和重复性良好，可为益母草配方颗粒质量控制提供参考。　　[关键词]益母草； 标准汤剂； 盐酸益母草碱； 盐酸水苏碱； 指纹图谱　　Standard decoction of Leonuri Herba　　DENG Zhe1，2， ZHANG Fan3， ZHANG Jun1， ZHANG Qing1， JIAO Mengjiao1， ZHANG Guoyuan1，　　CHENG Jintang1， LIU An1， WANG Yuesheng1*　　　　[Abstract]To build an evaluation standard for quality of Leonuri Herba standard decoction 13 batches of Leonuri Herba standard decoction with different quality were prepared The contents of leonurine hydrochloride and stachydrine hydrochloride were determined； then the transfer rate and the extract rate were calculated and pH value was measured； and HPLC fingerprint method was established for analysis The results of 13 batches of samples revealed that the transfer rate of leonurine hydrochloride and stachydrine hydrochloride was 300%534% and 670%826%， respectively； the extract rate was 121%183% and the pH value was Moreover， 12 common chromatographic peaks were determined based on fingerprint by using Similarity Evaluation System for Chromatographic fingerprint of Traditional Chinese Medicine The similarity of 13 batches of samples was analyzed and compared， and the results showed that the similarity was higher than 09 In this study， the preparation method for Leonuri Herba decoction was standard， with high similarity in fingerprint， showing high precision， stability and repeatability in fingerprint analysis Thus， this study can provide a reference for the quality control of Leonuri Herba dispensing granules　　[Key words]Leonuri Herba； standard decoction； leonurine hydrochloride； stachydrine hydrochloride； fingerprint　　益母草?榇叫慰浦参镆婺覆?Leonurus japonicas Houtt的新鲜或干燥地上部分，具有活血调经、利尿消肿、清热解毒等功效，临床常用于月经不调、痛经闭经、产后恶露不尽、水肿尿少、疮疡肿毒等病症。因其疗效显著，享有“妇科要药”“经产良药”之美誉。除此