脑康对大鼠出血性脑卒中的保护作用.pdfVIP

脑康对大鼠出血性脑卒中的保护作用 柳 倩 潘 玉* (辽宁省沈阳市中医研究所 沈阳 110003) [提 要] 目的：研究脑康对大鼠出血性脑卒中的保护作用。方法：采用大鼠注入自体血形成出血性脑卒中 模型,观察大鼠死亡率、脑神经功能缺失状态及断头后张口喘气时间,测试出血性脑卒中大鼠脑组织中丙二醛 (MDA) 含量、超氧化物歧化酶 (T-SOD)及谷胱甘肽过氧化物还原酶 (GSH-Px)活力。结果：脑康能明显减轻大鼠神经功能障 碍,延长断头后大鼠张口喘气时间,降低脑组织中 MDA 含量,并能明显增强 T-SOD 和 GSH-Px 活力。结论：脑康对大 鼠出血性脑卒中有保护作用,机制可能与抗脂质过氧化反应有关。 [关键词] 脑康 出血性脑卒中 神经功能障碍 脂质过氧化 Protective Effect of Naokang on Hemorrhagic Cerebral Apoplexy of Rats Liu Qian,Pan Yu (Shenyan Institute of TCM, Shenyang 110003) Abstrct Objective ：To study the protective effects of Naokang on hemorrhagic cerebral apoplexy in rats. Methods ：The animal model of hemorrhagic cerebral apoplexy was produced by injected auto blood of rats. The dead rate of hemorrhagic cerebral apoplexy rats, the score of neurological deficits and the duration of gasping of rats after complete brain ischemia were observed. The content of malondiadehyde (MDA), the total superoxide dismutase (T-SOD)activities and glutathione peroxidase (GSH-Px) activities in brain tissue of rats were tested. Results ：Naokang dosed-dependently reduced the score of neurological deficits and the dead rate of hemorrhagic cerebral apoplexy rats. Naokang decreased the content of MDA in brain tissue, suppressed the reduction of SOD activities and GSH-Px activities in brain. Conclushon ： Naokang has protective effects against hemorrhagic cerebral apoplexy in rats probably through inhibiting free radical and subsequent lipid peroxidation. Key words Naokang；hemorrhagic cerebral apoplexy；neurological deficits；lipid peroxidation 出血性脑卒中(Hemorrhagic cerebral apoplexy, HCA) 间的影响,观察脑康对脂质过氧化反应的影响[3]。 又称脑出血,是临床常见病之一,其死亡率高,致残率高[1]。然 1 实验材料 而目前国内针对出血性脑卒中研究者相对较少,作者在以往 1.1 药品与试剂