脑内局部给药建立颞叶癫疒间模型的研究.PDFVIP

362 JC lin Neuro,l October 2006, V ol. 19, No. 5 汤继宏, 包仕尧, 张志琳 　　【】　　 (EP)。　 (KA )4μg /kg, 、。　KA 、、、、;、、, , 10 h。 1 ～3, Ⅱ ～Ⅳ。 EEG , 。 KA CA CA , 1 3 CA 。　KA EP, 、 3 EP, EP。 【】　;;; 【】R742. 1　　　【】A　　　【】1004-1648(2006)05-0362-03 Study on establishm ent of tem poral lobe epilepsy m odel by adm inistrating drug in brain region　TANG J-i hong, BAO Sh-iyao, ZHANG Zh-i lin. Children sHosp ita lAff ilia ted toSuzhou Universiyt, Suzhou 15003, Ch ina Abstrac:t Objective　To explore a better ethod to establish te poral lobe epilepsy odel by ad inistrating drug in brain region. M ethods　Kainic acid (KA )4 μg /kg was injected into ratshippoca pus by stereotactic operation. The ratsbehavior, EEG and pathological changeswere observed. Results　After the ratshippoca pus injectedwith KA, staring, we-tdog shakes, asticatory ove ent and clonus of li bs occurred successively. The seizureswereparoxys alwith rotation, unsettled state of ju p and tic of li bs. The ratsbehavior gradually recovered to nor al after 10 hours. Then the spontaneous seizure ( ostly rating 2 ～ 4) occurred 1 ～3 ti es every week. Cluster electric discharge, spikewaves and sharpwaveswere recorded in cerebral cortex. KA-treated rats could result in hippoca pal CA and CA fields neuronal degeneration and necrosis, especially significant neuron loss was 1 3 observed in the CA field of KA injected ipsilateral side. Conclusions　 Injection KA in brain region of rat can 3 establish te poral epilepsy ode.l The sy pto , electrophysiology and pathological changes of te poral lobe epilepsy in the rat odel are al ost the sa e as those in hu an being. The KA induced rat odel is an ideal tool to research hu an te poral lobe epilepsy.