培养基对小麦单倍体胚成苗率的影响.PDFVIP

中国农学通报 2015,31(21): 107-110 Chinese Agricultural Science Bulletin 培养基对小麦单倍体胚成苗率的影响 高 艳，唐建卫，殷贵鸿，韩玉林，黄 峰，王丽娜，于海飞， 李楠楠，吕永军，张 倩，李顺成，杨光宇，李新平 （周口市农业科学院/河南省小麦种质改良工程技术研究中心，河南周口466000 ） 摘 要：为筛选出适宜小麦单倍体幼胚生长的培养基，提高小麦单倍体胚成苗率，将小麦×玉米远缘杂交 得到的小麦单倍体幼胚接种在8 种培养基上，每种培养基上接种2 个材料，平均值用于数据分析。8 种 培养基中幼苗平均产生率分别为 ：46.07% 、45.90% 、34.11% 、41.15% 、37.99% 、36.91% 、37.70% 和 52.51% 。结果表明1/2MS + 1.0 mg/L KT+3.0 mg/L 多效唑+ 100 mg/L 肌醇+5%蔗糖+0.6%琼脂，pH 5.8 培 养的幼胚成苗率最高，白化苗率低，且单倍体苗的长势较好，有利于培育壮苗。以1/2MS 为基本培养基， 加入微量的多效唑、KT 、肌醇以及较高浓度的蔗糖可以有效促进幼胚生长。 关键词：小麦；单倍体；培养基；激素；成苗率 中图分类号：S512.1 文献标志码：A 论文编号：cas EffectofCultureMediaonSeedlingRateofWheatHaploid GaoYan,TangJianwei,YinGuihong,HanYulin,HuangFeng,WangLina,YuHaifei,LiNannan, LvYongjun,ZhangQian,LiShuncheng,YangGuangyu,LiXinping (ZhoukouCityAcademyofAgriculturalSciences/WheatGermplasmImprovementEngineeringResearchCenterof HenanProvince,ZhoukouHenan466000) Abstract:Inordertoselecttheoptimalculturemediumforthegrowthofwheathaploidimmatureembryoand improvetheseedlingrate,wheathaploidimmatureembryosderivedfromwheat×maizecrosswereinoculated ineightmediums.Eachmediumwasinoculatedtwomaterials.Meanvaluesofthetwomaterialswereusedfor dataanalysis.Thefrequencyofseedlingproductionineightmediumswere46.07%,45.90%,34.11%,41.15%, 37.99%,36.91%,37.70%and52.51%respectively.Theresultsshowedthattheoptimumculturemediumwas pH5.8mediumof 1/2MS, 1.0 mg/LKT, 3.0 mg/LPP333, 100mg/Linose, 5%sucroseand0.6%agar.Inthe optimumculturemedium,theimmatureembryoseedlingratewasthehighest,albinoseedlingratewaslowand thehaploidplantletsgrewwell.Thebasicculturemediumof 1/2MS,addedwithtraceamountofPP333,KT, inositolandhighconcentrationsucrose,caneffectivelypromotetheembryogrowth.