用荧光染色法对柱状田头菇Agrocybeaegerita-南京农业大学学报.PDF

南京农业大学学报 　2000 , 23 (3) : 57～60 　 Journal of Nanjing Agricultural University 用荧光染色法对柱状田头菇 ( Agrocybe aegerita) 子实体担子和担孢子的观察 鲍大鹏 　王 　南 　谭 　琦 　潘迎捷 (上海市农业科学院食用菌研究所 , 农业部食用菌遗传育种重点开放实验室 , 上海市农业遗传育种重点实验室 , 上海 201106) 摘要 　运用荧光染色方法对 4 株不同地域来源的柱状田头菇子实体的担子和担孢子进行了观察。结果显示 , 来自欧洲希 腊的 Ag8 、中国四川省的Ag10 和台湾省的 AgT 菌株的有性繁殖结构特征是 4 孢双核 ; 而来自贵州省的 Ag9 菌株的有性繁 殖结构特征是双孢 4 核。这种 4 核现象首次在蕈菌中观察到。 关键词 　柱状田头菇 ; 担子 ; 担孢子 ; 荧光染色 分类号 　 Observation on basidia and basidiospores of Agrocybe aegerita by fluorescence staining Bao Dapeng , Wang Nan , Tan Qi and Pan Yingjie ( Key Lab of Edible Fungi Genetics and Breeding , Agricultural Ministry of China , Key Lab of Agriculture Genetics and Breeding of Shanghai , Edible Fungi Institute , Shanghai Academy of Agricultural Sciences , Shanghai 201106) ABSTRACT 　Basidia and basidiospores of A . aegerita strains from different areas were observed by fluorescence staining and electronic microscope. The results showed that the structural characteristics of sexual propagation of Ag8 (from Greece) , Ag10 (from Sichuan , China) and AgT (from Taiwan , China) is four basidiospores per basidium and two nuclei per basidospore. The structure in Ag9 (from Guizhou , China) is two basidiospores per basidium and four nuclei per basidiospore. The latter phenomenon was reported in cultivated mushroom for the first time. Key words 　Agrocybe aegerita ; basidium ; basidiospore ; fluorescence staining 柱状田头菇是一种不可多得的、具有极大开发潜力的食用菌新品种 , 又是一种适于作为栽培蕈菌的 模式种和进行生理生化及遗传学方面研究的模式菌株。国内外学者对柱状田头菇进行了多方面的研究。 欧洲和日本的研究者认为柱状田头菇有性繁殖类型是属于四极性的异宗结合[1 ] ,