响应面分析法优化微波辅助提取剑麻果胶的工艺.PDF

56 2013, Vol.34, No.18 食品科学 ※工艺技术 响应面分析法优化微波辅助提取剑麻 果胶的工艺 1,2 1 3 1 1,* 姚先超 ，张雪红 ，梁玉石 ，周 红 ，林翠梧 (1.广西大学化学化工学院，广西 南宁 530004 ；2.广西民族大学化学化工学院，广西 南宁 530006 ； 3.广西民族大学设备实验管理处，广西 南宁 530006) 摘  要：以剑麻纤维生产过程中产生的废渣为原料，采用微波辅助技术从中提取果胶。首先通过单因素试验考察选 取影响因素和相关水平，然后采用三因素三水平的响应面法分析设计优化微波功率、提取时间、溶液pH值等因素 的组合试验，对果胶提取条件进行优化和显著性分析，依据回归分析确定最佳提取工艺条件为微波功率534W 、提 取时间9.8min 、pH1.3 ，在此工艺条件下，剑麻果胶的提取率为6.10% ，其半乳糖醛酸含量和酯化度分别为75.3%和 72.09% 。 关键词：响应面分析法；剑麻；果胶；微波；提取 Optimization of Microwave-Assisted Extraction of Pectic Polysaccharides from Agave sisalana Perrine by Response Surface Methodology 1,2 1 3 1 1,* YAO Xian-chao ，ZHANG Xue-hong ，LIANG Yu-shi ，ZHOU Hong ，LIN Cui-wu (1. School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China； 2. School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China； 3. Department of Facility and Laboratory Management, Guangxi University for Nationalities, Nanning 530006, China) Abstract ：The microwave-assisted extraction of pectic polysaccharides from sisal wastes left over after fiber production was investigated. Response surface methodology (RSM) based on a three-level, three-variable Box-Behnken design was applied to optimize three extraction conditions including microwave output power, extraction time and solvent pH. The optimum extraction conditions were found to be 534 W, 9.8 min and pH 1.3. The yield of ASP (pectic from Agave sisalana Perrine) under the