健康妇女阴道乳杆菌的分离及鉴定-妇产科学专业论文.docxVIP

兰州大学硕士学位论文Isolation 兰州大学硕士学位论文 Isolation and Identification of Lactobacillus from healthy women’S vaginas Abstract objective Isolating lactobacillus from healthy female genital tracts，for exploring probiotics to research compound preparation．And explored a variety of therapeutic treatment of female reproductive system diseases theoretical basis for doing the preparatory work to use recombinant lactic acid bacteria． Methods Using traditional morphology analysis to identify lactobacillus，Where a gram-positive，catalase test Was negative，rod-shaped morphology of the strains could be initially identified as Lactobacillus．And ideniified as the members of the genus Lactobacillus by growth temperature(1 5℃，45℃)，motility test，nitrate reduction test, gelatin liquefaction test，indole(indole)test，hydrogen sulfide tests．Carbohy’drate fermentation test for species identification of Lactobacillus．Screening for hydrogen peroxide-producing lactobacillus．Applying 1 6S rRNA sequence analysis molecular biology methods to identify it exactly．And then examing predominant strian by performance test．These included：growth experiments，different incubation time changes in broth pH，temperature sensitivity of strains testing，acid—resistant strains of the initial screening of Lactobacillus in vitro antagonism tests，antibiotic sensitivity experiments，the thermal stability test metabolites．In addition，detecting strains of the advantages of plasmid． Results There were 1 1 strains of lactobacillus isolated and identifled from 1 8 health examination women genital tract，the detection rate was 6 1．1％．5 Lactobacillus species from 1 1 strains of lactobacillus were identified．4 were Lactobacillus acidlophilus，3 were Lactobacillus gassed，2 were Lactobacillus jensenii，1 was Lactobacillus cfispatus and another one Was Lactobacillus salivaius．Orgy one Lactobacillus WaS hydrogen peroxide—producing．Obtained the predominant strian and Lactobacillus crispatus the hi．ghest homology by molecular biology techni