增强UV-B辐射对棉花幼苗氮代谢生理特性的影响-中国棉花杂志社.PDF

花 学 报 Cotton Science 20 13 25 渊6 冤院525~532 增 UV-B 辐射对棉花幼苗氮代谢生理特性的影响 王 燕 茂营 张明才 谭伟明 段留生* 植物生长调节剂教育部工程研究 心/ 中国农业大学农学与生物技术学院袁北京100193 冤 摘要:在室内水培条件下袁人工模拟紫外辐射 Ultraviolet-B 冤袁研究UV-B 辐射对两个转Bt 基因抗虫棉材料 29317 系和29312 系幼苗物质积累和氮代谢生理特性的影响遥 实验设置3 个处理袁UV-B 辐射剂量分别是0 kJ 窑m-2 -1 -2 -1 -2 -1 窑d 尧2.7 kJ 窑m 窑d 尧5.4 kJ 窑m 窑d 遥 结果表明袁增强UV-B 辐射 以降低棉花幼苗地上部生物量的积累而 对地下部没有显著影响曰低强度的UV-B 辐射 2.7 kJ 窑m-2 窑d-1冤对 氮的含量没有影响袁而高强度的UV-B 辐射 5.4 kJ 窑m-2 窑d-1冤 以显著降低 氮的含量曰UV-B 辐射 以增加棉花幼苗叶片 硝酸还原酶(NR) 尧谷氨酰胺合 成酶(GS)尧蛋白酶尧肽酶的活性袁提高 溶性蛋白和游离氨基酸的含量遥UV-B 辐射提高棉花幼苗叶片 蛋白质 的合成和降解速度袁使氮代谢加强遥 棉花幼苗通过加速氮循环途径来适应UV-B 辐射袁降低伤害程度遥 关键词院UV-B 辐射曰棉花曰幼苗曰氮代谢 中图分类号院S562.01 文献标志码院A 文章编号院1002-7807 2013冤06-0525-08 WANG Yan, LI Mao-ying, ZHANG Ming-cai, TAN Wei-ming, DUAN Liu-sheng* ( 100193 ) The effects of ultraviolet-B (UV-B) radiation on the physiological characteristics of nitrogen metabolism were exam- ined in two transgenic Bt cotton cultivars, 29317 and 29312, under hydroponic conditions. UV-B was artificially applied via fil- tered lamps at three levels: 0 (CK), 2.7 and 5.4 kJ 窑m-2 -1 窑d . Our results showed that UV-B radiation decreased shoot dry weight, but had no impact on root dry weight accumulation. At 2.7 kJ 窑m-2 窑d-1 UV-B radiation, no significant difference in total nitrogen content compared with the control was observed. However, high levels of UV-B radiation at 5.4 kJ 窑m-2 窑d-1 significantly in- creased the total nitrogen content. Further physiological studies revealed that UV-B radiation upregulated nitrate reductase activ- ity, glutamine synthetase activity, protease activity, and peptidase activity. Higher contents of soluble protein and total free amino acids were induced by UV-B radiation. The result