基于近红外技术的金银花药材多指标成分快速检测-中国现代应用药学.PDF

基于近红外技术的金银花药材多指标成分快速检测  * 耿姝，陈勇，金叶，栾连军，刘雪松，吴永江 (浙江大学药学院，杭州 310058) 摘要：目的 采用近红外光谱(NIR)漫反射法和化学计量学方法对金银花药材质控指标绿原酸和木犀草苷的含量同时进行 快速检测，实现金银花药材质量的高效评价。方法 HPLC 分别测定不同批次金银花药材中绿原酸和木犀草苷成分的含 量，同时采集药材的 NIR 光谱，最终采用偏最小二乘回归(PLSR)法分别建立 NIR 与绿原酸和木犀草苷含量间的定量校正 模型，并对预测集样品中指标成分的含量进行预测。结果 所建 PLSR 定量模型的相关系数均0.90 ，RMSECV 分别为 0.469 和 0.012 4 ；采用独立验证集对模型进行外部验证，RMSEP 分别为 0.478 和 0.010 5 ，与 RMSEC 值相近且与 RMSECV 的比值接近于 1，RSEP 分别为 10.19%和 14.76%。所建 NIR 快速检测方法的准确度和精密度结果良好。结论 该分析方 法简便高效，可应用于不同批次金银花药材中绿原酸和木犀草苷 2 个关键质控指标含量的快速检测及质量控制，结果准 确可靠。 关键词：近红外光谱；金银花药材；绿原酸；木犀草苷；偏最小二乘回归法；快速检测 中图分类号：R284.1 ；R917.101 文献标志码：B 文章编号：1007-7693(2016)02-0144-07 DOI: 10.13748/ki.issn1007-7693.2016.02.003 Rapid Quantitative Determination of Multi-Indicators in Lonicerae Japonicae Flos by Near-Infrared Spectroscopy * GENG Shu, CHEN Yong, JIN Ye, LUAN Lianjun, LIU Xuesong, WU Yongjiang (College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China) ABSTRACT: OBJECTIVE To establish a set of quantitative calibration models based on near-infrared (NIR) spectroscopy and chemometrics methods for rapid determination of chlorogenic acid and luteoloside content in Lonicerae Japonicae Flos at the same time, which is useful for the quality control process. METHODS The PLSR algorithm was finally used to build NIR quantitative models between the spectra and the content of chlorogenic acid and luteoloside determined by the reference methods. The validation set was used to identify the predictive performance of the established models. RESULTS For PLSR models, the correlation coefficients of