棉花黄萎病拮抗菌株DS42的分离鉴定-河南农业科学.PDF

DO :10.15933/j .cnki .1004 -3268.2010.01.005 20 10 年第1 期 DS45-2 ＊ 陈　妍, 李红亚, 李术娜, 王　全, 王　伟, 王树香, 朱宝成 ( , 07 100 1) :从土样中筛选对棉花黄萎病致病菌大丽轮枝菌(Vert ici ll ium dah l iae Kleb)具有拮抗作用的 芽孢杆菌菌株, 初筛得 具有拮抗作用的菌株 68 株, 复筛选出1 株拮抗活性较高的菌株DS45-2, 通过形态特征观察, 生理生化试验及16S rDNA 序列分析对此菌株进行鉴定, 初步鉴定此菌株属于 解淀粉芽孢杆菌(B aci l lus amy lo liquef aciens ), 与相应标准菌株 NBRC 15535 的16S rDNA 序列 相似度达99 .50%。 :大丽轮枝菌;拮抗细菌;分离;鉴定;解淀粉芽孢杆菌 :S435.621.2+4 　　:A 　　:1004-3268 (2010 )01-0068-05 solation and dentification of Antag onistic Strain DS45-2 against Co tt on Vertici lli um Wilt CH EN Yan, L H ong-ya, L Shu-na, WA NG Quan, WA NG Wei, ＊ WA NG Shu-xiang, Z H U Bao-cheng (College of Bio lo gy, A g ricultur al U nive rsity of H ebei, Bao ding 07 1001, China) Abstract:T he strains w it h ant agonistic act ivity against Ver ti ci l li um dah li ae Kleb.were screened f ro m the soil.Of t he 68 strains obt ained, a strain named DS45-2 w ith a relatively hig her antago - nistic act ivity .T he strain DS45-2 w as furt her purified and characterized by m orpho logical and cul- ture observatio n, phy siolog ical and biochemical experiment s and 16S rDNA sequence analy sis. Based on t he result s, t he strain w as initially identified as a B aci ll us amy lo li quef aciens .T he sim i- la rity of t he 16S rDNA sequences between strain DS45-2 and t he ty pe strain NBRC 15535 of B a - ci l lus amy lol iquef aciens w as close to 99.50%. Key words:Ver tici l li um dah l iae Kleb;A ntago nistic bact eria; solatio n; dentif icatio