探讨中草药水萃物对中枢神经细胞CB1表现及动物抗焦虑行为之影响.DOCVIP

PAGE \* MERGEFORMAT 1 探討中草藥水萃物對中樞神經細胞CB1表現及動物抗焦慮行為之影響 Effects of Water Extracts of Chinese Herbs on the CB1 Expression on Central Nervous System and Anti-anxiety Behavior in Vivo 研究生：陳和豊 Ho-Li Chen 指導教授：莊秀琪 Hso-Chi Chaung 【摘要】 焦慮是一種常見的病症，易導致腦部海馬迴神經系統之細胞損傷。本實驗探討茯神、柏子仁、五加皮、酸棗仁四種中草藥水萃取物對於中樞神經細胞活性及抗焦慮行為之影響，及其對Cannabinol receptor 1 (CB1)表現之作用。體外試驗以中草藥水萃取物作用於C6 大鼠神經膠瘤細胞株，並以H2O2造成氧化壓力，以評估此四種中草藥水萃取物是否具有保護細胞之效果。動物試驗則以中草藥水萃取物各別添加於ICR小鼠飲用水中，再以監禁誘發小鼠產生焦慮之動物模式，進行高架十字迷宮測試其焦慮程度，並取其海馬迴組織，以流式細胞儀偵測神經細胞及星狀神經細胞CB1受體之表現量及細胞之存活率，以分析中草藥水萃取物對於中樞神經之保護作用。實驗結果顯示酸棗仁可提高C6細胞存活率 (P 0.01)，而動物試驗結果顯示五加皮與酸棗仁之水萃取物可增加實驗動物於十字迷宮開放臂的次數與時間 (p 0.05) ，顯示其具有抗焦慮作用，且海馬迴組織中神經細胞和星狀神經膠細胞之CB1受體表現量和細胞存活率與RO飲用水控制組相比有顯著增加。因此，五加皮、酸棗仁可有效改善動物之焦慮行為，並可保護中樞神經系統細胞活性，此作用可能與CB1表現量有關。 關鍵字：茯神、柏子仁、五加皮、酸棗仁、CB1受體、高架十字迷宮、海馬迴 【Abstract】 The anxiety disorders are the most common mental disorders, which can lead to hippocampal damage. This study is to examine the effects of water extracts of four Chinese herbal medicines, Poria Cocos (PC), Platycladi Semen (PS), Cortex Acanthopanacis Radicis (CAR) and Semen Zizyphi Spinosae (SZS), on glial cell activities in vitro and anti-anxiety and Cannabinol receptor 1 (CB1) expression in vivo. In the in vitro study, hydrogen peroxide was used to induce oxidative stress in C6 glioma cells. Results showed that SZS significantly increased cell viabilities (P 0.01) of C6 glial cells as compared with PBS control. In the in vivo experiment, the ICR mice were randomized into 5 groups : 4 treatments of water extracts of different Chinese herbal medicines and one of water control. The anxiety-like behaviors under restraint stress were evaluated by using the elevated plus-maze test. The apoptosis of neurons/astrocyte and CB1 expression were detected using flow cytometry. Results of the animal study revealed that CAR or SZS significantly increased the open-arm entries and time (p 0.05), CB1 receptor expression and cell viabilities of neurons and astrocytes in hippocampal. Hence, water extracts of C