真菌DNA条形码研究进展.PDF

菌物学报 jwxt@ 15 November 2012, 31(6): 809-820 Http:// Mycosystema ISSN1672-6472 CN11-5180/Q © 2012 IMCAS, all rights reserved. 真菌 DNA 条形码研究进展 1,2 2* 张宇 郭良栋 1 齐齐哈尔大学生命科学与工程学院 黑龙江 齐齐哈尔 161006 2 中国科学院微生物研究所真菌学国家重点实验室 北京 100101 摘 要：DNA 条形码（DNA barcode ）是通过一段短的标准DNA 片段实现物种的快速、准确和标准化鉴定。线粒体 细胞色素 C 氧化酶亚基 I （COI ）基因作为动物的DNA 条形码已广泛应用于物种鉴定中，在植物上已选定叶绿体 rbcL 和 matK 基因作为基本的 DNA 条形码。目前世界各国真菌学家正对不同的真菌类群进行不同基因片段的筛选与评价， 并在第四届国际生命条形码大会上正式推荐了 ITS 作为真菌的首选 DNA 条形码。对国内外真菌 DNA 条形码的研究 进展进行总结与分析，并展望真菌 DNA 条形码的应用前景。 关键词：真菌鉴定，DNA 条形码，基因，评价，ITS Progress of fungal DNA barcode ZHANG Yu1, 2 GUO Liang-Dong2* 1College of Life Science and Engineering, Qiqihar University, Qiqihar, Heilongjiang 161006, China 2State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China Abstract: DNA barcode uses a short gene sequence taken from standardized portions of the genome to identify species. Cytochrome oxidase I (COI), as an animal DNA barcode, has been successfully employed in the species identification. In plants a combination of chloroplast rbcL and matK genes has been accepted as basic DNA barcode. In fungi more genes have being screened and evaluated in all major lineages of fungi by mycologists all over the world. Recently, the internal transcribed spacer (ITS) has been recommended as primary DNA barcode of fungi in the Fourth International Barcode of Life Conference. This review summarized the recent progress of fungal DNA barcode, and pointed out the prospect of DNA barcode in future fungal studies. Key words: fungal identification, DNA barcode, gene, evaluation, ITS 基金项目：中国科学院重要方向性项目（No. KSCX2-YW-G-068 ）；国家自然科学基金（No.） * Corresponding author. E-mail: guold@ 收稿日期: 2011-11-25, 接受日期: 2011-12-20 810 ISSN1672-6472 CN11-5180/Q Mycosystema Nove