西伯利亚铁线莲地上部分有效成分分析及总黄酮的测定-中国管理科学.PDF

※工艺技术 食品科学 2009, Vol. 30, No. 24 221 古丽巴哈尔·阿巴拜克力 (新疆师范大学生命科学与化学学院生物系，新疆乌鲁木齐 830054) ：对西伯利亚铁线莲的地上部分采取不同的方法进行有效成分分析，即水提法、醇提法、石油醚提取法， 结果表明，西伯利亚铁线莲中含有丰富的有效成分，采用乙醇回流浸提法对西伯利亚铁线莲的不同部位进行了总黄 酮的提取和测定，结果总黄酮的含量顺序为叶＞果实＞茎。 ：西伯利亚铁线莲；成分；总黄酮；测定 Analysis of Active Ingredients and Total Flavonoids in Clematis sibirica (L.) Mill. GULIBAHAER Ababaikeli (Department of Biology, College of Life Science and Chemistry, Xinjiang Normal University, Urumqi 830054, China) Abstract ：The active ingredients from above-ground parts were prepared using aqueous extraction and solvent extraction including ethanol and petroleum ether. The performing condition for solvent extraction of flavonoids was optimized by an orthogonal experiment design and the factors influencing the yields of flavonoids were also investigated including extraction time, temperature, solvent concentration and liquid/solid ratio. The results suggested the abundance of active ingredients in above- ground part of Clematis sibirica (L.) Mill. Analyses on the contents of flavonoids in the extracts from various parts of Clematis sibirica (L.) Mill showed that all the parts were rich in flavonoids while the contents varied and the order decreased as follows: leaf fruit stalk. Key words ：Clematis sibirica (L.) Mill.；active ingredients ；total flavonoids ；determination 中图分类号：R931.71 文献标识码：A 文章编号：1002-6630(2009)24-0221-06 西伯利亚铁线莲Clematis sibirica (L.) Mill. 属于毛茛 等均为分析纯。 科[ 1 ] 。有关西伯利亚铁线莲有效成分研究尚未见报道。 1.2 仪器与设备 本实验针对新疆西伯利亚铁线莲分布广等特点[2] ，对其 RE-5 旋转蒸发器 上海亚荣生化仪器厂；PL303-IC 不同部位的有效成分进行较为系统的定性分析，并对 电子天平(感量0.0001g) 上海梅特勒- 托利多仪器有限公 其不同部位进行总黄酮提取工艺研究，以期为西伯