高效液相色谱法测定发酵乳中的乳糖葡萄糖和半乳糖.PDF

162 2012, Vol. 33, No. 04 食品科学 ※分析检测 李 琦，张兰威 * ，张英春，易华西，杜 明，韩 雪，郭春锋，李婧妍，薛超辉 (哈尔滨工业大学食品科学与工程学院，黑龙江 哈尔滨 150090) 利用高效液相色谱法测定发酵乳中乳糖、葡萄糖、半乳糖的含量。样品通过 Carrez 法处理沉淀蛋白，采 用 Aminex HPX 87H 色谱柱， 5mmol/L H2 SO4 溶液为流动相，流速 0.6mL/min 。结果表明：利用该法进行色谱分析 各组分达到较好的分离，各糖组分在 0.5～12mg/mL 范围内呈现良好线性关系，相关系数为 0.9986～0.9999(n ＝ 5) ， 样品回收率为 96.45%～101.78%；精密度实验表明，各组分峰面积RSD 在 0.62%～4.21% 之间(n ＝ 5) ；重现性实 验表明，各组分含量测定值 RSD 为 1.86%～3.44% 。该法测定样品快速、准确、可靠，为发酵乳贮藏过程中关 键糖含量的测定提供参考依据。 高效液相色谱法；发酵乳；乳糖；葡萄糖；半乳糖 Determination of Lactose, Glucose and Galactose Contents in Fermented Milk by HPLC LI Qi ，ZHANG Lan-wei* ，ZHANG Ying-chun ，YI Hua-xi ，DU Ming ，HAN Xue ， GUO Chun-feng ，LI Jing-yan ，XUE Chao-hui (School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China) Abstract：An HPLC method for the determination of lactose, glucose and galactose in fermented milk was established. Proteins from samples were precipitated with Carrez reagent. The chromatographic separation was achieved on an Aminex HPX 87H column using 5 mmol/L H2 SO4 as mobile phase at a flow rate of 0.6 mL/min. Good separation was obtained for the analytes. The calibration curves established showed good linearity over the concentration range of 0.5 to 12 mg/mL, with a correlation coefficient of 0.9986 to 0.9999 (n ＝5). The mean spike recoveries for the sugars in fermented skim milk prepared in our lab varied from 96.45% to 101.78% with a RSD between 0.62% and 4.21% (n ＝5). The reproducibility RSDs ranged from 1.86% to 3.44%. The method was rapid, accurate and reliable. Key words ：high pressure liquid chromatography (HPLC)；fermented milk ；lacose ；glucose ；galactose 中图分类号：O657.72 文献标识码：A 文章编号：10