尖端电喷雾萃取电离质谱技术在食管癌诊断中的应用-心胸外科专业论文.docxVIP

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尖端电喷雾萃取电离质谱技术在食管癌诊断中的应用-心胸外科专业论文.docx

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目录目录万方数据万方数据目录第 1 章前言··············································································································1 第 2 章材料与方法·································································································· 3 2.1 材料·············································································································3 2.1.1 研究对象·························································································· 3 2.1.2 标本保存及处理···············································································4 2.1.3 主要仪器与试剂···············································································5 2.2 实验方法····································································································· 5 HYPERLINK \l _TOC_250003 Tip-Spray 离子源的平台搭建： 5 HYPERLINK \l _TOC_250002 Tip-Spray 离子源的参数设置： 7 HYPERLINK \l _TOC_250001 组织样品的 Tip-Spray-MS 检测： 7 HYPERLINK \l _TOC_250000 2.3 Tip-Spray-MS 指纹谱图的统计学分析： 7 第 3 章结果··············································································································9 3.1 食管癌及癌旁组织质谱指纹图谱分析······················································ 9 3.2 主成分分析································································································ 10 3.2.1 主成分分析的初步结果································································· 10 3.2.2 各组主成分的贡献值对比····························································· 10 3.2.3 各组主成分内对应的质核比··························································11 3.3 串联质谱碰撞诱导解离及物质成分分析··················································12 第 4 章讨论············································································································14 4.1 食管癌快速诊断技术的研发···································································· 14 4.2 质谱技术在食管癌诊断中的独特地位·····················································14 4.3 尖端电喷雾萃取电离质谱技术对食管癌的诊断·································