简化的人纤溶酶原饼环区5基因的克隆及表达与初步鉴定-生物化学与分子生物学专业论文.docxVIP

简化的人纤溶酶原饼环区5基因的克隆及表达与初步鉴定English 简化的人纤溶酶原饼环区5基因的克隆及表达与初步鉴定 English abstract ● Cloning and Fusion Expression of Predigested Human Plasminogen Kringle 5 in Escherichia coli Master candidate：Wang Jianqi Supervisor：Zhai Chaoyang BaekgroundObjective Angiogenesis happens in many pathological processes，such嬲diabetic minopathy,cardiovaScular disorders。tamor ● growth,incursion，and metastaSis．Briefly speaking,angiogenesis is regulated by both positive signals and negative signals．Among the reported endogenesis negative regulators，angiostatin is one of the most effective inhibitor,which is all internal fragment of plasminogen and consisted by the first four kringle domams of plasminogen．It has been documented that angiostatin could inhibit tunlor growth effectively／n vivo and in vitro．Further more，it WaS reported recently that the fifth kringle domam conjointed with angiostatin by a lysine WaS human plasminogen kringie 5(hPK一5)，which had more potem ability of inhibition of migration of endothelial cells and neovascularization induced by growth factor and ev雌inducement of apoptosis of some CanCer cells．Smdy in molecular genetics shows m吨these ● tangle domains display high structure similarity and about 500,6 sequence identity to each other．But their biological activity is different，intact kringlel-3 haS potent activity on inhibiting proliferation of endothelial cells， 简化的人纤溶酶原饼环区5基因的克隆及表达与初步鉴定while 简化的人纤溶酶原饼环区5基因的克隆及表达与初步鉴定 while K4 has a marginal effect．K5 works well in both migration and ● proliferation of endothelial cells，and that makes kringle 5 to have more significant study value and application in clinic．Up to date,getting hPK-5 is mainly by recombinant technology but the process is SO complex and the small quantity of the recombined still has large molecular weight correspondingly．So we deleted some amino acids both from N—side and C-side,and kept the three disulfide bonds inside via pre-designed primers．And we direetly cloned predigested hPK-5 and co—express