窖蛋白1与饮食诱导肥胖及相关心血管疾病的关系-营养与食品卫生学专业论文.docxVIP

华中科技大学同济医学院 华中科技大学同济医学院 硕：I：学位论文 余各指标之间差异无统计学意义(P0．05)。 结论：①高脂饲料可诱导SD大鼠发生肥胖，对肥胖大鼠进行低脂饮食干预 可减少脂肪的蓄积，有利于控制肥胖发展的趋势。②肥胖大鼠中窖蛋白一1表达升 高、eNOS表达降低，可导致内皮功能失调从而增加心血管疾病的危险眭。③肥胖大 鼠改喂低脂饮食可降低血管caveolin．1的表达，并恢复eNOS的蛋白水平，通过 改善内皮功能降低心血管疾病的危险性。 创新点：目前国内外对窖蛋白．1的研究主要集中在对内皮功能的调节及其与 冠心病的关系方面，而饮食诱导肥胖对窖蛋白一1的影响，尤其是膳食干预对肥胖 大鼠窖蛋白一1及其下游eNOS表达的影响尚未见相关报道，因而本研究在国内外 具有一定程度的创新性。 关键词：高脂饮食，肥胖，窖蛋白．1，内皮型一氧化氮合酶， 华中科技大学同济医学院 华中科技大学同济医学院 坝lj学位论文 The Relationship between Caveolin—l and Dietary Induced Obesity and cardiovascular disease in Rats Master Candidate：Mingjia Xu Tutor：Associate Professor Nianhong Yang Associate Professor Chenjiang Ying Abstract Objective To study the effect of high—fat diet on obesity in rats and the effect of dietary fat on the expression ofcaveolin一1，endothelial nitricoxide synthase(eNOS)in artery of rats Reveal the possible mechanisms of diet induced obesity and cardiovascular diseases，and provide evidences for preventing obesity and cardiovascular diseases． Methods Thirty·six male SPF Sprague·Dawley rats(1 509～1 609)were randomly divided into high-fat diet group(HF,n227)and control group(CF,n29)on the basis of body weight after acclimated to the environment and chow diet for 1 week．Then either high-fat or chow diet were given for fifteen weeks．The dietary induced obesity (DIO)rats were designated from HF group according to the final body weight fweights more than i+1 96s of control group)．Then half of the DIO rats were switched to standard diet(DIO-HF／LF)，the other DIO rats(DIO—HF)and CF rats continued their primary diet for another eight weeks．Blood samples were collected by caudal vein for the measurement of fast blood glucose and blood lipid levels at the beginning of the test and before the DIO rats were switched with different diets． General conditions were observed daily，and body weights were measured weekly At the end of the experiment by the 2