胶质瘤干细胞壁龛的实验研究-外科学(神外)专业论文.docxVIP

安徽医科大学硕士学位论文 安徽医科大学硕士学位论文 5 5 immunohistochemistry.There were positive correlation between the CD133+/Ki-67+cells or Nestin+/Ki-67+cells with CD133+/Nestin+BTSCs.Furthermore,besides CD133+/Nestin+BTSCs, there are also subgroup cells ,such as CD133+/Nestin-cells or CD133-/PCNA+cells in the niches,. (3) In low-group, there were fewer CD133+blood vessels or Nestin+ blood vessels in the regions which CD133+niches or Nestin+niches distributied compared with the high-group. The CD133+/CD31+,Nestin+/CD31+cells could be found co-expressed in the endothelial cells by using double immunofluorescence staining. Positive correlation was observed between the CD133+ niches and CD133+blood vessels （r =0.945,P0.01）which similar to the correlation between the Nestin+ blood vessels and Nestin+ niches（r =0.727,P0.01）. Conclusion (1) Along with the tumor grading increased,the BTSCs niches,the shape and structural elements of which are changing unceasingly,increased significantly. (2) Along with the tumor grading increased,both the BTSCs and subgroup cells in the niches were at the state of proliferating and differentiating.(3) The BTSCs niches could be found distributed around the micro-vascular system with strong correlation. Moreover, we also found the blood vessels in the niches as a structural ingredient.Therefore, the BTSCs niches and the micro-vascular system are related to each other closely, which were showed not only in the regional distribution but also in the biological function. Key Words Glioma;Brain tumor stem cells;niche;Immunohistochemistry; Microvasculary;CD133；Nestin 1 1 英文缩略词表 英文缩写 BTSCs 英文全称 Brain tumor stem cells 中文名称 脑肿瘤干细胞 NSCs Neural stem sells 神经干细胞 GBM Glioblastoma 胶质母细胞瘤 PBS Phosphate buffered solution 磷酸盐缓冲液 DAPI Diamidine-phenyl-indolel 二脒基苯基吲哚 SVZ subventricular zone 脑室下区 SGZ subgranular zone 海马结构的颗粒下区 PCNA Proliferating Cell nuclear 增殖细胞核抗原 FITC Fluorescein isothiocyanate 异硫氰酸荧光素 HIF-1α ECM hypoxia inducible factor-1α extracellular ma