接骨八味散对陈旧性骨折愈合过程中BMP及VEGF表达的影响-医学专业论文.docxVIP

万方数据 万方数据 和VEGF高峰出现在第35天，D组BMP和VEGF高峰出现在第35天； 结论：使用安全有效剂量的接骨八味散内服，能加快骨折修复细胞的分化、增殖， 增加成骨细胞的活性，加快骨折局部骨痂的堆积，诱导骨折局部BMP和VEGF的合成，使 BMP和VEGF的峰值提前，加速大鼠创伤性陈旧性骨折的愈合，提高陈旧性骨折愈合质量， 预防陈旧性骨折骨不连或迟缓愈合现象的发生。 【关键词】接骨八味散；陈旧性骨折；骨折愈合；血管内皮生长因子；骨发生形态蛋白 3 ABSTRACT Bawei scattered on the expression of VEGF and BMP in the old bone fracture healing process and BMP expression Objective To observe the dispersion of Bawei bone fracture healing process of old bone tissue vascular endothelial growth factor (VEGF) and bone morphogenetic protein (BMP-2) expression as well as the fracture callus stump value of CT analysis of its efforts to promote the healing of old fracture mechanism. Methods 72 Wistar rats were randomly divided into normal group and model group, model group was divided into one of the control group and treatment group. Artificial methods of bone caused by folding the middle of wild rats with fractures of the right femur fracture model standards. A group of (non-surgical group) 16, B group (14 days after fracture fixation add saline gavage) 16, C group (14 days after the fracture fixation plate add bulk Bawei gavage) 16, D group (21 days after fracture fixation add saline gavage) 12, E group (21 days after fracture fixation osteosynthesis add Bawei San ig) 12. After fracture at 14 days, 21 days at different time points to B, C, D, E groups open reduction and internal fixation surgery and after the B, D ig saline group, C, E Group Bawei bone San gavage, And after at different time points (after fracture 14,21,35,49 d) Acquisition fracture value of CT samples measured, HE staining, immunohistochemical method callus determination of vascular endothelial growth factor VEGF and bone morphogenetic protein BMP -2 the number of positive cells, and distribution of Change. Results 1. CT values: the first 14 days of the CT value of A group and B group was no significant difference, B group and C group was no significant difference; the first 21 days of the CT value of B group was significantl