化学遗传学筛选斑马鱼突变体突变基因的定位讲座.pptxVIP

化学遗传学筛选应用：斑马鱼突变体突变基因的定位 Positional cloning (mapping) of mutant;Large-scale Forward Genetic Screening （2008-） NR/SBB/βe1/rag1;;;350 mutant: The expressions of mpx and lyz are not affected , but MPO is enzymatically inactive ;;350 mutant: Primitive hematopoiesis is not affected;36hpf;Phenotype;;Molecular markers for BSA: RFLPs(Restriction Fragment Length Polymorphisms) RAPDs(Random Amplified Polymorphism DNAs) SSLPs(Simple Sequence Length Polymorphisms);;z10919 on chromosome 11 appeared to be linked with 350 mutational site;;;;;;z3362;z10215;z10919;350 mutational site is close to the north end of chromosome 11;z10919;CU633745-3;;Exon sequence:;A highly conserved Poc1a in zebrafish;SOFT (short stature, onychodysplasia, facial dysmorphism, and hypotrichosis);poc1a cas9