接骨方对骨愈合中骨连接素mRNA表达影响的动物实验与临床研究-中医骨伤科学专业论文.docxVIP

骨折愈合疗效方面，治疗组总有效率为95．7％，与对照组(总有效率90．9％)相比， 骨折愈合疗效方面，治疗组总有效率为95．7％，与对照组(总有效率90．9％)相比， 统计学上无显著性差异(PO．05)。 结论： 根据本次实验结果，以及对骨折愈合的病因病机认识，并回顾中药接骨方以往的 研究，经分析得出如下结论：1)本实验采用标准化的闭合性二期骨折愈合模型，是较 为理想的实验研究动物模型。2)中药接骨方能明显地改善骨折后疼痛、肿胀、皮肤 瘀斑、骨折局部压痛、纵叩痛及骨痂情况的作用，提高骨折愈合疗效，促进骨折愈合。 3)接骨方可能通过激活成骨细胞、成软骨细胞的活性，促进成骨细胞增殖、分化、 矿化、骨形成功能，发挥其促进骨折愈合的作用，此外，它对调节软骨细胞的增殖也 可能有作用。4)在分子水平上，中药接骨方通过促进ON mRNA的提前大量表达，使 软骨修复提早进入骨化及塑形期，从而促进骨折修复，加强骨折愈合。 关键词： 骨折愈合； 原位杂交； 骨连接素 The The Effect of J i egufang on Osteonect i n mRNA i n Fracture HeaI ing Course though the AnimaI’s Experiment and the C1．nicaI Research SpeciaI ity：Bone Traumatology of Traditional Chinese Medicine Author：He yanhui Tutor：Prof．Wang Bingnan Abstract Objective To investigate the effect of Jiegufang on ON in mRNA gene expression in the process of fracture healing though the animal’S experiment and clinical research，and to explore the mechanism of Jiegufang in enhancing fracture heal ing． methods： The animal’s experiment： forty female SD rats were produced by operation a closed-fracture experimental model in the right middle femoral， were divided equally into two groups(treatment and contr01)at random，rats in treatment group were taken with Jiegufang liquid by 10．4mL per kilogram,while in control group were taken with the same volume isotonic Na chloride．Animals in two groups were killed respectively after operation of 2w and 4w．Took out the callus in sterile circumstance and kept them frozen in Liquid Nitrogen．firstly，to observe the variance and differentiation of callus disposed with different measurements at different phases by chemistry staining，which included routine paraffin section，and HE taining．Secondly，The expression of the mRNA for ON was investigated by in situ hybridization technique during the healing process of experimental fracture． The clinical research： 48 radius fracture patients(2006．9～2007．2)were randomly divided into treatment group and control group．Treatment group were given Jiegufang and shangkejiegupian，