茎尖嫁接脱除柑橘黄龙病病原及其检测方法比较.PDFVIP

2006,35(4):24-27. Subtropical Plant Science 茎尖嫁接脱除柑橘黄龙病病原及其检测方法比较 1,2 1 1 刘柏玲 ，李国怀 ，程云清 (1.华中农业大学 园艺林学学院，湖北 武汉 430070 ；2. 曲阜师范大学 生命科学学院，山东 曲阜 273165) 摘 要：运用茎尖微芽嫁接技术脱除柑橘黄龙病病原，并采用 PCR 技术和直接荧光法对脱毒后的植株进行脱 毒效率检测。结果表明，茎尖微芽嫁接成活率最高为 75%；茎尖微芽嫁接技术是脱除黄龙病病原的一种有效 方法，脱毒率达 100%，脱毒后均检测不到病原存在；PCR 技术检测病毒的检出率为 100%，而直接荧光法的 检出率仅为 33.3% ，说明PCR 技术具有更高的灵敏度。 关键词：柑橘；茎尖微芽嫁接；黄龙病；PCR；直接荧光法 中图分类号：S41-30; S432.2+5; S436.661.1 文献标识码：A 文章编号：1009-7791(2006)04-0024-04 Elimination of Citrus Huanglongbing Diseases by Shoot-tip Grafting and the Methods of Detection 1,2 1 1 LIU Bo-ling , LI Guo-huai , CHENG Yun-qing (1.College of Horticulture and Forestry Science, Huazhong Agriculture University, Wuhan 430070, Hubei China; 2.College of Life Science, Qufu Normal University, Qufu 273165, Shandong China) Abstract: The shoot-tip grafting (STG) technique was used to eliminate the bacterial-like organisms (BLOs), and survival plants were obtained by the technique. Polymerase chain reaction (PCR) and direct fluorescence detection methods were regarded to detect the efficiency of STG. The result showed that the highest rate of survival plant was 75%; the BLOs were not detected by the two methods in the STG plants, while the original material all infected by BLOs. The efficiency of elimination of huanglongbing by STG was 100%; the rate of detection by PCR was 100%, but only 33.3% by direct fluorescence detection. So PCR has higher sensitivity. These young STG plants obtained by STG were virus-free young plants and STG could be used for propagating virus-fr