金柑果皮油胞特性和相关调控基因表达量的研究-果树学专业论文.docxVIP

脆蜜金柑比较、滑皮金柑和脆蜜金柑比较分别有12个、28个、22个显著性富集 脆蜜金柑比较、滑皮金柑和脆蜜金柑比较分别有12个、28个、22个显著性富集 的GOterm。 6．通过与NR库比对，200条基因编码了超过46种不同类别的转录因子，参与 烯类等物质调控有关的AP2／ERF和WRKY转录因子，在融安金柑和滑皮金柑比 较中有AP2／ERF转录因子4个；在融安金柑和脆蜜金柑比较中，WRKY转录因 子26个，在滑皮金柑和脆蜜金柑比较中，有WRKY转录因子18个。 7．筛选获得135条DEGs可能编码油胞精油成分烯萜类、酮类物质的5条调控 酶基因和5条转录因子。预测金柑油胞相关物质的控制基因可能是在类黄酮生物 合成途径中，控制柚皮素一3．双加氧酶促进柚皮素、二氢槲皮素等物质合成的酶 基因Unigene0008513和调控新薄荷醇脱氢酶促进薄荷醇合成的Unigene0000766 酶基因。 关键词：脆蜜金柑；油胞；高通量测序；表达量分析 II 万方数据 Study Study on the characteristics of peel oil cell and the expression of oil cell associated gene on Kumquat GaoXing (College ofAgriculture，Guangxi University，Nanning 530004，China) Abstract Kumquat is the fruit can be eaten with skin among citrus fruits．The size，amount and inclusion of oil vacuole on pericarp can directly affect the edible quality． Therefore，for the sake of the kumquat oil vacuole，this experiment will take a research on the fruit quality as well as the differences of oil vacuole’S size and amount through taking common kumquat Rongan with more oil vacuole，kumquat Huapi in bud mutation with less oil vacuole and kumquat Cuimi in bud mutation with least oil vacuole as the research materials．These three varieties of fruit transcriptome shall be sequenced and then through research on the differential gene，the relative controlling gene affecting the formation of oil vacuole can be analyzed．Besides，taking advantage of the expression quantity of measured selecting gen in each stage，the various expressions for these gens in the development process of fruit Can be analyzed What’S more，the connection between theses gens and the development process of oil vacuole Can be predicated．It aims at providing a theoretical foundation for the cultivation of kumquat in high quality with less essential oil in oil vacuole in the applied genetic engineering． 1．The number of oil cells have shown from initial growth among three kumquats． Within the fruit enlaring，the density of oil cell decreases slightly． In accordance with the amount of oil vacuole