金匮肾气丸对雄性环磷酰胺大鼠生殖功能的影响-中医外科学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 PAGE PAGE 10 中 文 摘 要 目的：探讨金匮肾气丸对腹腔注射环磷酰胺（CTX）雄性 SD 大鼠生殖功能的影响 及部分作用机理。 方法：取 15 月龄雄性 SD 大鼠 40 只，随机分成 4 组：模型组，金匮肾气丸低剂 量组、中剂量组、高剂量组，每组均 10 只。每组大鼠均给予腹腔注射环磷酰胺 混悬液（1ml/kg·d－1，1 次/d）连续 5d；模型组以蒸馏水灌胃，金匮肾气丸低 剂量组、中剂量组、高剂量组分别给予金匮肾气丸混悬液低剂量、中剂量、高剂 量灌胃，连续 28 天。每天观察并记录大鼠活动情况、体重及精神状态变化；末 次给药 24 小时处死各组大鼠，测睾丸附睾的湿重；取附睾液镜下观察精子密度、 活力和活率；光镜结合图像分析系统观察睾丸的显微结构；测血清睾酮(T)。 结果：自用环磷酰胺第 4 天起，各组大鼠均出现体重减轻，活动减少，反应迟钝， 体毛卷曲、发黄、干枯无光泽易脱落，尾巴湿冷等类似肾阳虚的症状；而金匮肾 气丸各组可改善上述症状及表现；实验结束，用药组与模型组比较，在睾丸附睾 脏器系数、精子密度、精子活力和活率、睾丸曲细精管直径及间质细胞数量、血 清睾酮均有所提高，有统计学意义。 结论：环磷酰胺染毒能复制 SD 大鼠肾阳虚和生殖功能低下模型，致使大鼠生殖 能力下降，金匮肾气丸可以提高环磷酰胺染毒大鼠的睾丸附睾脏器系数；提高环 磷酰胺染毒大鼠精液精子密度、活力和活率；提高环磷酰胺染毒大鼠睾丸曲细精 管直径和间质细胞的的数量；提高环磷酰胺染毒大鼠血清睾酮水平。得出金匮肾 气丸可以有效拮抗环磷酰胺对 SD 大鼠的生殖毒性，保护其生殖功能。 关键词：金匮肾气丸；生精功能障碍；睾酮；不育 ABSTRACT Objective: To explore the effect and mechanism of the Jinkui Shenqi Pill on the genetic function of male SD rats with CTX. Method: Divide the 40 male SD rats, which are averagely 15 months old, into four groups randomly: model group, treated group with low dosage Jinkui Shenqi Pill, treated group with middle dosage,treated group with high dosage，Each group has 10 rats. Each group were injected into the the abdominal cavity with Cyclophosphamide(1ml/kg?d-1 ) for 5 continuous days；the model group were Gavaged with NS for 28 continuous days and the high、the middle、the low dosage group were Gavaged with Jinkui Shenqi Pill suspl of the high、the middle、the low dosage for 28 continuous days. Every time,we observed the changes of rats in the activities and mental status.At last,we killed the rats of each group in 24 hours, and calculated the weight of the testicle and epididymis, observed the density and motility rate of the Epididymal fluid, and observed the microstructure of the testicle under optical microscope with image analysis system,measured the Serum testosterone. Result: Due to the Cyclophosphamide，the rats would lose weight, decrease activities and become slowness. Its pelage would become yellow and parch blight, Its tail