金克对乳腺癌裸鼠移植瘤模型生长转移和凋亡的影响-外科学(普外)专业论文.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 PAGE PAGE 10 金克对乳腺癌裸鼠移植瘤模型生长转移和凋亡的影响 硕士研究生 袁 鹏 导 师 黄 韬 教 授 摘 要 目的 观察金克（槐耳清膏）对人乳腺癌裸鼠移植瘤的抑制作用及其对肿瘤细胞增殖 和凋亡的影响。 方法 以 MCF-7 细胞复制裸鼠异种移植瘤模型，30 只荷瘤鼠随机分组用药：对照组、 金克组。分别用生理盐水和金克连续灌胃 30d，测量瘤体大小，记下生存期，观察各 组的抑瘤效果及对裸鼠一般状况、瘤体大小和生存时间的影响。收集肿瘤标本，采用 光镜、Tunel 法行原位凋亡检测、流式细胞术观察凋亡的形态学变化及细胞周期的改 变。 结果 ① 金克组瘤体积大小明显小于对照组的（4.62±1.86 cm3 和 6.35±2.73 cm3， P 0.01）; ② 金克组生存期为明显长于对照组的（116.7±28.5d 和 95.3±26.7d， P0.01）; ③原位凋亡检测移植瘤细胞凋亡指数（AI）明显高于对照组的，差异有显 著性（19.32%和 8.36%，P0.01）；④ 流式细胞术显示金克组凋亡比例与对照组差异 有显著性（18.21%和 8.39%，P0.01） 结论 金克可抑制人乳腺癌细胞 MCF-7 移植瘤生长，延长生存期。 关键词 金克；乳腺癌；移植瘤；凋亡 The effect of Jinke on growth transfer and apoptosis of transplantable tumor model in nude mice with breast cancer Medical master Yuan Peng Tutor Huang Tao Professor Abstract Objective To investigate the effects of Jinke on the tumour growth, cell proliferation and apoptosis in transplantable tumor of breast cancer cell line MCF-7 in nude mice. Methods MCF-7 cells were inoculated into the mammary fatty pad of nude mice to establish breast cancer model, then thirty BALB/c nude mice with xenograft tumor were randomized into two groups: Control group and Jinke group. The Jinke group was given Jinke while control one was given normal saline water by gastrogavage in 0.2ml volume for 30 days. The effects of Jinke on the tumor growth, the tumour volume and survival days were observed and recorded. Samples of tumor were collected. The sections of tumor were observed under light microscope. Cell apoptosis in situ was examined by a Tunel assay. The morphological variation of apoptosis was observed through immunohisto-chemical staining method. Results 1 The tumor volume of Control group were significantly larger than that of Jinke group（4.62±1.86 cm3 和 6.35±2.73 cm3，P 0.01）. 2 The survival days of Jinke group were significantly longer than in Control group （116.7±28.5d 和 95.3±26.7d，P0.01）. 3 Detection of apoptosis in situ showed that apoptosis index (AI) was significantly higher in Jinke group than in Contro