一株高产木聚糖酶的枝链霉菌的分离鉴定及产酶.PDF

微生物学通报 JUN 20, 2010, 37(6): 791−797 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 一株高产木聚糖酶的枝链霉菌的分离鉴定及产酶 * 李秀婷 孙宝国 宋焕禄 吕跃钢 佘元莉 宋红霞 (北京工商大学化学与环境工程学院 北京 100048) 摘 要: 对 1 株高产木聚糖酶的链霉菌进行了鉴定并研究其木聚糖酶的生产过程及水解产物特点。 分离得到 1 株产木聚糖酶的链霉菌Streptomyces sp. L2001, 从形态学特征、培养特征和生理生化特 征等方面对该菌株进行了鉴定。PCR 扩增得到 16S rDNA 序列全长为 1429 bp, 分析结果表明, 菌株 与Streptomyces rameus NBRC3782 同源性达99.16%。结合传统生理生化实验结果鉴定为枝链霉菌。 菌株液体发酵6 d 能产生842.0 U/mL 木聚糖酶活力。经HPLC 分析酶解产物, 结果显示木二糖、木 三糖及木四糖含量之和高达93.5%, 该酶适用于工业化生产低聚木糖。 关键词: 枝链霉菌, 16S rRNA, 鉴定, 木聚糖酶 Isolation, Indentification of a Streptomyces rameus Strain with High Level Xylanase Activity and Its Enzyme Production * LI Xiu-Ting SUN Bao-Guo SONG Huan-Lu LV Yue-Gang SHE Yuan-Li SONG Hong-Xia (School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100048, China) Abstract: A Streptomyces rameus strain with high yeild in xylanase activity was identified. Its capac- ity to produce xylanase and the characteristics of its hydrolysates were also investigated. A new strain of Streptomyces sp. L2001 was isolated from soil samples and was identified using morphological, cultural, physiological and biochemical characterisctics. The total length of 16S rDNA fragment was 1429 bp throu