酒精中毒后的血液荧光光谱研究-光学工程专业论文.docxVIP

硕士论文 硕士论文 酒精中毒后的血液荧光光谱研究 摘 要 本文以新西兰白兔灌注白酒制作成不同程度酒精中毒模型，用多功能光栅光谱仪 ● 检测醉酒白兔血液的荧光光谱，进而从理论上分析和解释了荧光光谱峰产生的机制。 研究了波长为407rim的非相干单色光激发严重酒精中毒的兔全血、红细胞混悬 液，其荧光光谱的检测结果表明：随着酒精中毒出现昏迷到生物体征逐渐恢复的过程 中，同一浓度的全血、红细胞溶液的荧光强度都发生了规律性的变化。分析认为酒精 中毒引起红细胞的体积增大，并增加了血液的粘度。前者能增加分子间碰撞几率，引 起荧光淬灭；而后者却能增加辐射跃迁的几率，使得荧光增强。在全血和红细胞溶液 中，荧光光谱的变化规律是两者共同作用的结果．在同样方法和实验条件下得到了一 般酒精中毒兔全血、红细胞混悬液的荧光光谱，结果表明：从酒精中毒至慢慢恢复的 过程中，同一浓度的全血、红细胞溶液，其荧光强度的变化规律与严重酒精中毒时一 致，只是变化幅度较小。 研究了波长为407rim的相干激光激发不同浓度的、严重酒精中毒的全血溶液的 荧光光谱，结果表明：酒精中毒后的全血荧光光谱的峰值、强度与溶液浓度间均有较 ’ 强的依赖关系，进而利用能量转移理论解释了其产生机制。 本文的研究结果可以为研究酒精中毒情况下生物体征的损伤和血液的破坏程度 ■ 以及能否恢复、血液中酒精含量的判定等提供实验和理论依据。 关键词：血液，酒精中毒，荧光光谱，荧光猝灭 ■ 硕士论文 硕士论文 酒精中毒后的血液荧光光谱研究 ABSTRACT Based on the experimental research on the alcoholism models of different degrees by ● making New Zealand rabbits drink distilled spirit and the test on the fluorescence spectra of drunk rabbits with the multifunctional grating spectra instrument,the article theoretically analyzes and explained the system why the fluorescence spectra peak comes into being． Through the experiments on the serious exanimation alcoholism blood offluorescence spectra ofwhole blood and erythrocyte induced by 407nm Xe lamp，it is shown that during the course when rabbits change from uncomcious state to conscious state，the intensity of alcoholism blood of fluorescence spectra of whole blood and erythrocyte of the same concentration changes regularly．h is considered that the alcoholism causes the enlarging of the volume of erythrocyte勰well as the increase of viscidity tO blood．11硷former Can increase the probability of the collisions between the blood cells，and thus causes the fluorescence quenching；while the latter eaEt increase the probability of the change of radiation,and consequently increases the spectra．Both of thcm contribute to the changing rules of the fluorescence spectra in the mixture of whole blood and erythrocyte．With the same method and under the s锄e condition,we get the bibulosity．alcohol