基于microRNAmRNA配对表达谱进行联合分析的方法学进展.docx

基于microRNA-mRNA配对表达谱进行联 合分析的方法学进展 赵路阳张康顾成磊叶明侠范文生韩为东孟元 解放军总医院妇产科北京华信医院妇产科解放军 第309医院妇产科解放军总医院基础研究所 摘要： microRNA (miRNA)是一类内源性非编码小RNA,主要通过降解靶基因或抑制靶 基因的翻译而调控表达。由于其作用机制复杂，目前尚未发现高效而低成本的靶 标定位方法。近年来，基于碱基互补配对原理的计算机预测法被广为应用，但此 方法假阳性高，不同算法所得结果差界大，会误导和干扰下游的功能学实验。因 此，有研究者提出结合样木配对mRNA表达量来进一步定位靶基因，明确 miRNA-mRNA相互作用方式，这种联合分析的方法受到了普遍认可。本文冋顾了 近年来基于miRNA-mRNA配对表达谱进行联合分析的方法学进展，并简要分析各 类方法的应用范围和优缺点，为后续研究者选择方法提供参考。 关键词： microRNA; microRNA 靶标预测;microRNA-mRNA 相互作用; 联合分析; 作者简介：赵路阳(1988-)，女，博士研究生，(E-mail) zlynfyd@163. com 作者简介：孟元光，(E-mail) mcng6512@vip. sina.. com 收稿日期:2016-12-22 基金：国家自然科学基金面上项目 Progress in the Integrative Analysis of microRNA and mRNA Expression Data ZHAO Lu-Yang ZHANG Kang GU Cheng-Lei YE Ming-Xia FAN Wen-Sheng HAN Wei-Dong MENG Yuan-Guang Department of Gynecology and Obstetties, Chinese PLA General Hopsitai; Department of Gynecology and Obstetrics, Beijing Huaxin Hospital; Institute of Basic Medicine, Chinese PLA General Hopsital; Abstract： microRNAs (miRNAs) are endogenous non-coding small RNAs that interact with their mRNAs by degrading or inhibiting translation of the targets. Up to date, there is still no low-cost and effective miRNA target screening method because the regulatory mechanisms are complex. In recent years, some investigators built several computational methods based on sequence complementarity^ of the miRNA and the mRNAs. However, the results are various out of different algorithms and have huge rate of false positives, which caused great troubles in downstream experiments. Therefore, methods of using the expression values of miRNAs and mRNAs to refine the resuIts has been proposed, which have shown to effeetivcly identifying the most prominent int eractions. Here, we summarized t hese met hods that combining both miRNA expression values and mRXA expression values and to predict miRNA targets, outlined the advantages and disadvantages of different methods, and provided directions for future investigation. Keyword： microRNA; microRNA target predi