PCDHα在髓鞘形成和少突胶质细胞发育中的作用.PDFVIP

动 物 学 研 究 2012，Aug. 33(4): 362−366 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI ：10.3724/SP.J.1141.2012.04362 PCDHα在髓鞘形成和少突胶质细胞发育中的作用 于 钰，索 伦，吴 强* (上海交通大学系统生物医学研究院, 上海 200240) 摘要：该文通过免疫组化及蛋白免疫印迹的方法分别对Pcdhα基因敲除和对照组小鼠的中枢神经系统内的髓 鞘碱性蛋白表达以及少突胶质细胞的发育进行了测定。结果表明：1) Pcdhα基因缺失小鼠中枢神经系统中的髓鞘 碱性蛋白较对照组小鼠明显减少；2) Pcdhα , 基因敲除可导致少突胶质细胞发育异常：在小脑中 处于成熟期的少 , Pcdhα 突胶质细胞减少 而处于前体细胞阶段的少突胶质细胞增多。上述结果提示 可以通过调控少突胶质细胞的 成熟过程进而影响髓鞘的形成。 关键词：原钙黏蛋白；髓鞘；少突胶质细胞；发育 中图分类号：Q952.5; Q42 文献标志码：A 文章编号：0254-5853-(2012)04-0362-05 Protocadherin α gene cluster is required for myelination and oligodendrocyte development YU Yu, SUO Lun, WU Qiang* (Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China) Abstract: This work used Immunohistochemistry to examine the expression of myelin basic protein and accumulation of oligodendrocytes in Pchdα knockout and control littermate mice. Data showed that in Pchdα knockout mice, Myelin proteins decrease in the central nervous system and mature oligodendrocytes in the cerebellum also decrease. Furthermore, deletion of the Pcdhα cluster does not cause any change to the axons and astrocytes in quantification of relative marker proteins. These findings suggest that the Pcdhα cluster may be required for myelination and oligodendrite development of the brain in mice, and that Pcdhα cluster may play a key role in the development of the central nervous system. Key words: Prot