利用纤维床反应器固定化发酵生产丙酸-微生物学报.PDF

生 物 工 程 学 报 Chin J Biotech 2008, June 25; 24(6): 1075-1079 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2008 Institute of Microbiology, CAS CSM, All rights reserved 研究报告 冯小海, 吴波, 沈晓波, 徐虹 , 210009 : 构建了一种纤维床反应器(FBB), 并将其应用于丙酸的生产。将棉纤维绕成桶状, 固定于反应器中, 即可用于丙 酸固定化发酵。以40 g/L 的葡萄糖为碳源, 与游离细胞相比, 利用FBB 生产丙酸, 丙酸产量由14.58 g/L提高至20.41 g/L, 发酵时间由 120 h 缩短至60 h 。研究了不同糖浓度条件下FBB 生产丙酸情况, 并将补料策略应用于丙酸发酵中。结果 表明: 补料发酵能够有效改善Propionibacterium freudenreichii CCTCC M207015 在高糖条件下丙酸对葡萄糖转化率较 低、副产物较多的问题。经补料发酵280 h, 丙酸产量达45.91 g/L, 丙酸质量约占有机酸总质量比例为72.31% 。 : 纤维床反应器, Propionibacterium freudenreichii CCTCC M207015, 丙酸, 补料发酵 Propionic Acid Fermentation by Propionibacterium freudenreichii CCTCC M207015 with a Fibrous-bed Bioreactor Xiaohai Feng, Bo Wu, Xiaobo Shen, and Hong Xu State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, China Abstract: The production of propionic acid by Propionibacterium freudenreichii CCTCC M207015 was investigated in a Fibrous-bed bioreactor (FBB). The FBB was constructed by packing spiral cotton fibrous and immobilized into a bioreactor. By applying this bioreactor to propionic acid fermentation, the propionic acid yield had a significant improvement and reached 20.41 g/L, compared with the cell-free culture of 14.58 g/L (40 g/L of glucose). At the same time, the glucose exhausting time decreased from 120 h to 60 h. Batch fermentations at various glucose concent