河南工业大学优秀学位论文培育项目.doc

PAGE \* MERGEFORMAT1 叔丁基对苯二酚及其氧化产物与牛血清蛋白的相互作用 李军 毕艳兰 孙尚德 丁一冉 （河南工业大学粮油食品学院，河南郑州 450001） 摘要 采用紫外吸收光谱和荧光光谱法研究叔丁基对苯二酚（TBHQ）及其氧化产物叔丁基对苯二醌（TQ）与牛血清蛋白（BSA）的结合作用。结果表明，TBHQ和TQ与BSA之间发生了相互作用并形成新的聚合体；TBHQ和TQ与BSA的荧光猝灭均属于静态猝灭机制。TBHQ和TQ与BSA之间的结合常数均大于104 L/mol，表明它们之间形成的配合物结合较为牢固；TBHQ和TQ与BSA之间的结合位点数均为1；通过热力学参数方程计算得出：ΔH0、ΔS0和ΔG0，表明它们之间结合的主要作用力是氢键和疏水作用力，此过程是自发进行的。根据Forster非辐射能量转移理论计算出供能体（BSA）与受能体（TBHQ和TQ）之间的结合距离r分别为3.13 nm和3.73 nm。紫外吸收光谱和同步荧光光谱的研究结果表明TBHQ和TQ与BSA的结合引起了BSA的构象变化。 关键词 叔丁基对苯二酚 叔丁基对苯二醌 牛血清蛋白 紫外吸收光谱 荧光光谱 中图分类号：O657 文献标识码：A 文章编号：1003-0174（ ） Interaction of Tertiary Butylhydroquinone and Its Oxidation Product with Bovine Serum Albumin Li Jun Bi Yanlan Sun Shangde Ding Yiran (School of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001) Abstract The interactions of tertiary butylhydroquinone (TBHQ) and its oxidation product—2-tert-butyl-1, 4-benzoquinone (TQ) with bovine serum albumin (BSA) were investigated by ultraviolet (UV) spectrophotometry and spectrofluorimetry. The experimental results showed that the interactions of TBHQ and TQ with BSA occurred and new polymers were formed. The quenching mechanisms of BSA by TBHQ and TQ were static procedures. Both binding constants involved in the interaction between TBHQ/TQ and BSA were above 104 L/mol, which indicated that the combinations of TBHQ and TQ with BSA was very firm. The number of both binding sites involved in the interaction between TBHQ/TQ and BSA was 1. Their thermodynamic parameters were also evaluated and showed that ΔH was negative value and ΔS was positive value, which indicated that hydrogen bonding and hydrophobic interactions played major roles in the binding between BSA and TBHQ/TQ. ΔG was negative value, which indicated that the interaction process was spontaneous. Based on the Forster’s theory of non-radiation energy transfer, the binding distances (r) between the donor (BSA) and acceptor (TBHQ or TQ) were 3.13 nm and 3.73 nm, respectively. The results of ult