大蒜素对TRAIL诱导卵巢癌细胞SKOV-3凋亡影响的体外实验研究.pdf

英文摘要 into PRMI1640mediaaddedwith experimentalgroup(basic control PRMI1640 50mg／L only)，after allicin)andgroup(basic 24hthecellswerecollectedandthentested of expressions TRAIL DR4andDR5FCM recepetors by through was 3timesandthemeandata assay index(FI)．Eachperformed areavailable． 4 beforeandafterallicintreatment： Caspaseactivityassay in in SKOV-3cells wereseededcultureflasks logarithmicphase ata of inculturemedium．After 4．5x104cells／ml density having overin cellsweredividedinto stayed 48h，the PRMI with 1640mediaadded 50mg／L group(basic allicin)and control PRMI1640 weretested OD405 group(basic only)and by of and8activity spectrophotometer．ThechangesCaspase-3 theratiosof was showed ODallicin／ODblank．Each by assay 3timesandthemeandataareavaliable． performed 5 ofTRAIL and DR4，DR5 Expressions receptors beforeandafterallicintreatment Caspase-3，8