番茄红素卢一环化酶基因三班启动子调控L啪-华南师范大学学报.PDF

华南师范大学学报(自然科学版) 2016，48(4)：50—56 JournalofSouthChinaNormal Science University(NaturalEdition) 番茄红素卢一环化酶基因(三班)启动子调控 L啪RNAi双元载体构建 莫爱琼，文 了，黎海燕，马 丽，万小荣+ (仲恺农业工程学院生命科学学院，广州510225) 534 se，LcyB)基因起始密码子上游1bp启动子区域序列(LcyBp)，生物信息学分析表明，该启动子序列中存在TA- TA一盒、CAAT一盒、昼夜节律响应元件Circadian、光响应元件BoxI、真菌激发子响应元件Box．W1、低温响应元件 据番茄LcyB基因序列，设计2对含有不同酶切位点的特异引物迸行PCR扩增k徊基因3’端特异的276bpDNA 术特异性敲除LcyB基因进而提高番茄果实中番茄红素含量奠定实验基础． 关键词：番茄；番茄红素J8一环化酶(Lycopene口一cyclase，LcyB)；启动子；RNAi双元载体 中图分类号：Q945．1 文献标志码：A 文章编号：1000—5463(2016)04—0050—07 ConstructionofRNAi VectortoSilence Gene Binary Lycopene卢-cyclase DrivenNative Promoter LcyB by LcyB M0 Aiqiong，WENLiao，LIHaiyan，MALi，WANXiaorong+ ofLife of and 5 (CollegeSciences，ZhongkaiUniversityA鲥cultureEngineering，Guangzhou10225，China) the of the Abstract：Suppressingexpressiongeneencodinglycopene／3一cyclase(LcyB)，thekeyenzymeregulating metabolismof aneffectivetoincreasethecontentof the lycopeneto卢一carotene，is way lycopene．Inpresentstudy，a 1 534 of of was from clonedMicro—Tom bppromoter5’upstreamLcyBgene tomato(Lycopersicon inthedatabaseof resultshowedthat bioinformaticallyanalyzed plantcis—actingregulatoryelement(PlantCARE)．The several were elementsidentifiedinthe the importantcis—acting pro