苹果单倍体原生质体培养再生植株.PDF

( ) 核 农 学 报 　2004 ,18 6 :411～415 4 11 　 Acta Agriculturae N ucleatae Sinica ( ) 文章编号 2004 0641105 苹果单倍体原生质体培养再生植株 1 2 何道一 　孙 　山 ( 1 淮北煤炭师范学院生物系 ,安徽 淮北 　235000 ;2 山东省果树研究所 , 山东 泰安 　271000) 摘 　要 : 以富士单倍体试管苗幼叶和幼叶诱导的愈伤组织为材料制备原生质体 ,采用不同培养 方法获得原生质体愈伤组织 ,在分化培养基上成功地诱导原生质体来源的愈伤组织再生不定 芽 。试验结果显示试管苗幼叶诱导的愈伤组织是制备高质量原生质体的良好材料 ,每 g 愈伤 6 组织可制备原生质体 43 ×10 个 ;海藻酸钠球培养法是苹果原生质体培养的适宜方法 ,最终 植板率达 15 % ;在分化培养基中添加精氨酸能有效提高愈伤组织不定芽分化能力 ,原生质体 来源的愈伤组织分化频率高达 88 % 。 关键词 :苹果 ;单倍体 ;原生质体 ;植株再生 PLANT REGENERATION FROM PROTOPLASTS OF HAPLOID IND UCED BY IRRADIATION IN APPL E 1 2 HE Daoyi 　SUN Shan ( 1. Dep artment of B iology , Huaibei Coal Teacher College , Huaibei ,A nhui , 235000 ; 2 . Shandong Institute of Pomology , Tai ’an , Shandong , 271000) Abstract :Protoplasts were prepared from young leaves and callus derived from young leaves of Fuj i haploid shoots in vitro . Protoplast callus was recovered under different culture protocols and bud regeneration was accomplished from the protoplast callus. The results suggested that callus derived from the young leaves was suitable materials for prep 6 aration of protoplasts and 43 ×10 protoplasts were obtained from 1 g callus. Among 3 different culture protocols , protoplasts embedded in sodium alginate bead have the highest FPE of 15 % , regeneration rate was up to 88 %. The regeneration rate was significantly increased when Arg was added