雌激素新兴污染物於河川底泥中吸附特性之探讨.DOC

雌激素新興污染物於河川底泥中吸附特性之探討 Sorption of Estrogens on River Sediments 研究生：林玉婕 Lin, Yu-Chieh 指導教授：葉桂君 Yeh, Kuei-Jyum 【摘要】 雌激素（estrogens）有高內分泌干擾特性，且在環境中濃度漸增。利用固相萃取法（solid-phase extraction, SPE） 搭配電噴灑離子化（electrospray ionization, ESI）界面之液相層析串聯質譜儀（liquid chromatography-tandem mass spectrometry, LC-MS-MS）檢測水樣中雌二醇（17β-estradiol, E2）、雌激酮（estrone, E1）、雌三醇 （estriol, E3）、乙炔基雌二醇（17α-ethynylestrodiol , EE2）和己烯雌酚（diethylstilbestrol, DES）等五種天然與人工合成之雌激素類新興污染物。結果顯示使用SampliQ OPT 固相萃取管柱，試驗5 ng/L 分析物之回收率探討，各分析物之回收率均高於82%。為了解雌激素吸附特性，本研究挑選四種有機質差異大之河川底泥進行吸附試驗，其五種雌激素化合物大部分符合線性吸附模式（R2＞0.75）。有機質含量雖為影響吸附重要因素之一，但有機質成分與底泥基本性質亦為影響底泥-水分配係數（log Koc）之重要因子。本研究結果顯示，雌激素之辛醇-水分配係數（log Kow）與底泥-水分配係數（log Koc） 呈正比關係；基本性質相似之河川底泥，其吸附特性亦同。 關鍵字：雌激素化合物、LC-MS-MS、固相萃取（SPE）、底泥-水分配比（log Koc） 【Abstract】 Estrogens, which are the most potent endocrine-disrupting chemicals（EDCs）,can alter or disrupt normal hormonal functions through their mimicking of the behavior. Estrogens have hecome a worldwide concern because their concentrations in the environment have increased. This study developed a reliable and sensitive analytical method by combining solid-phase extraction（SPE）with liquid chromatography-tandem mass spectrometry（LC-MS-MS）for determining five estrogenic contaminants, 17β-estradiol (E2), estrone (E1), estriol (E3), 17α-ethyny- lestrodiol (EE2), and diethylstilbestrol (DES). Recovery of the estrogenic chemicals in spiked deionize water samples ranged from 82 to 99%. MDL of the estrogens ranged from 0.05 to 0.15 ng/L, which the method described here with great recovery and sensitivity.This study also evaluated the sorption behavior of estrogenic compounds in sediments in Kaohsiung and Pingtung areas. There sediments contained organic matter of wide range and different characteristics. The sorption of tested estrogen compounds were well correlated to linear sorption isotherm (R2＞0.75). The content and characteristics of sediment organic matters were two major factors for estrogen sorption. Furthermore, a linear correlat